| 环氧化硅胶固定木瓜蛋白酶及其动力学性质 |
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| 引用本文： | 刘海燕,孙素芳,杨更亮,李海鹰,王愈聪. 环氧化硅胶固定木瓜蛋白酶及其动力学性质[J]. 河北大学学报(自然科学版), 2002, 22(1): 40-43. DOI: 10.3969/j.issn.1000-1565.2002.01.011 |
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| 作者姓名： | 刘海燕 孙素芳 杨更亮 李海鹰 王愈聪 |
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| 作者单位： | 河北大学化学与环境科学学院,河北,保定,071002 |
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| 基金项目： | 国家自然科学基金资助项目 (2 0 0 75 0 0 5 )，河北省自然科学基金资助项目 (2 0 0 0 77) |
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| 摘 要： | 以硅胶为载体,γ-环氧丙基氧丙基三甲氧基硅烷为偶联剂,对木瓜蛋白酶进行了固定化.最适固定化条件如下:温度为25℃,pH为8.0,时间为18 h,酶量为每100mg环氧化硅胶固定24 mg酶.同时以酪蛋白为底物,研究了固定化条件对酶活力回收的影响,并比较了固定化酶和溶液酶的动力学性质,结果表明:固定化酶的最适pH和最适温度都比溶液酶有所提高,且有较好的操作稳定性.
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| 关 键 词： | 木瓜蛋白酶 环氧化硅胶 固定化酶 |
| 文章编号： | 1000-1565(2002)01-0040-04 |
| 修稿时间： | 2001-06-10 |
Study of the Immobilization of Papain on Epoxide - Activated Silica and Kinetic Properties of Immobilized Enzyme |
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| LIU Hai yan,SUN Su fang,YANG Geng liang,LI Hai ying,WANG Yu cong. Study of the Immobilization of Papain on Epoxide - Activated Silica and Kinetic Properties of Immobilized Enzyme[J]. Journal of Hebei University (Natural Science Edition), 2002, 22(1): 40-43. DOI: 10.3969/j.issn.1000-1565.2002.01.011 |
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| Authors: | LIU Hai yan SUN Su fang YANG Geng liang LI Hai ying WANG Yu cong |
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| Abstract: | Papain was immobilized on silica gel by γ-glycidoxypropyltrimethoxysilane. The optimum immobilization conditions of the enzyme were as follows: the temperature was 25 ℃, pH was 8.0,time was 18 h, and the amount of enzyme was 24 mg per 100 mg epoxide-activated silica. Some kinetic properties of the free and the immobilized enzyme were studied with casein as substrate. The results showed that the optimum pH and temperature for the immobilized enzyme were slight higher than those for the free one, and the thermal stability of the immobilized enzyme was better than that of the free. Meanwhile, the effect of some salts and organic solvents on the free and immobilized enzyme was also discussed. |
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| Keywords: | papain epoxide-activated silica immobilized enzyme |
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