| 不同品种香菇交配型基因的鉴定 |
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| 引用本文： | 叶明,汪咏梅,潘迎捷.不同品种香菇交配型基因的鉴定[J].安徽工程科技学院学报,2000,15(3):55-58. |
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| 作者姓名： | 叶明 汪咏梅 潘迎捷 |
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| 作者单位： | 1. 安徽机电学院生化工程系安徽芜湖 241000
2. 上海食用菌研究所农业部食用菌遗传育种重点开放实验室上海 201106 |
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| 摘 要： | 利用原生质法单核体亲本交配型极性标记法，成功地鉴定出香菇－栽培株（Ｓ）与２野生株 （Ｑ＆Ｈ）的 ４种孢子单核体标准交配型基因，并以具有交配型基因的香菇栽培株的 ４种孢子单核体 与香菇２野生株４种孢子单核体轮配，而鉴定出香菇２野生株的４种孢子单核体相对交配型基因， 为香菇的遗传研究提供了可靠的遗传标记．结果显示栽培株Ｓ的４种孢子单核体的交配型分别为 Ａ１Ｂ１、 Ａ２Ｂ２、 Ａ１Ｂ２与 Ａ２Ｂ１，野生株 Ｑ的 ４种孢子单核体的交配型为 Ａ３Ｂ３、 Ａ４Ｂ４、 Ａ３Ｂ４与 Ａ４Ｂ３，野生株 Ｈ４４种孢子单核体的交配型为Ａ５Ｂ５、Ａ６Ｂ６、Ａ５Ｂ６与Ａ６Ｂ５．
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| 关 键 词： | 香菇 交配型基因 亲本交配型极性标记法 鉴定 |
| 文章编号： | 1007-5240(2000)03-0055-04 |
| 修稿时间： | 2000年4月28日 |
The identification on mating genes of different breed in lentinula edodes |
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| YE Ming, WANG Yong-mei, PAN Ying-jie.The identification on mating genes of different breed in lentinula edodes[J].Journal of Anhui University of Technology and Science,2000,15(3):55-58. |
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| Authors: | YE Ming WANG Yong-mei PAN Ying-jie |
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| Abstract: | Four kinds of mating genes of spore monokaryons from cultivated strain (S) and two wild strains (Q&H) of Lentinula edodes Were identified successfully by the marker of parental mating type from protoplast monokaryon, and four kinds of spore monokaryons. The relative ones from two wild strains were done by the method. Four kinds of mating type genes of spore monokaryons from S mated with four ones from Q and H respectively. These provide the reliable and genetic markers for the genetic research of L. edodes. The result showed that mating types of four kinds of spore monokaryons from S is respectively A1B1, A2B2, A1B2 and A2B1; ones from Q are A3B3, A4B4, A3B4 and A4B3; and ones from H are A5B5, A6B6, A5B6 and A6B5. |
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| Keywords: | lentinula edodes mating type gene marker of parental mating type protoplast monokaryon identification |
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