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| 胃癌下调新基因GDDR表达特性及功能研究 | |
| 引用本文： | 褚光辉,杜建军,杨国栋,窦科峰,卢兹凡.胃癌下调新基因GDDR表达特性及功能研究[J].科学技术与工程,2009,9(7). |
| 作者姓名： | 褚光辉 杜建军 杨国栋 窦科峰 卢兹凡 |
| 作者单位： | 1. 第四军医大学西京消化病医院,西安,710032 2. 肿瘤生物学国家重点实验室生物化学与分子生物学教研室,西安,710032 3. 西京医院肝胆外科,西安,710032 |
| 摘 要： | 明确胃癌下调新基因GDDR的亚细胞定位、分泌特性及生物学功能研究.与绿色荧光蛋白(GFP)融合表达明确GDDR表达产物亚细胞定位;GES细胞系转染上清检测分泌蛋白表达;胃癌7901细胞系稳定转染GDDR,观察其生物学作用.发现GDDR表达产物定位于胞浆,是一分泌蛋白.四甲基偶氮唑盐比色法(MTT assay)检测描绘生长曲线及流式细胞计数仪(FACS)细胞周期检测表明,GDDR对胃癌7901细胞增值显著抑制(96 h(1.233±0.017) vs (0.618±0.015), t =2.447, P =2.63×10-9),并且可以引起细胞的周期阻滞.说明胃癌下调新基因GDDR产物为胞浆分布,具有分泌特性,能通过阻滞细胞周期显著抑制胃癌细胞生长,可能为新的候选抑癌基因. |
| 关 键 词： | 胃肿瘤 分泌蛋白 细胞周期 功能研究 |
Function Study of Novel Gene GDDR Differentially Expressed in Gastic Cancer |
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| CHU Guang-hui,DU Jian-jun,YANG Guo-dong,DOU Ke-feng,LU Zi-fan.Function Study of Novel Gene GDDR Differentially Expressed in Gastic Cancer[J].Science Technology and Engineering,2009,9(7). | |
| Authors: | CHU Guang-hui DU Jian-jun YANG Guo-dong DOU Ke-feng LU Zi-fan |
| Institution: | Xijing Hospital of Digestive Diseases;the Fourth Military Medical University;Department of Biochemistry and Molecular Biology;the State Key lab of cancer biology;the Fourth Military Medical University1;Department of Hepatobiliary Surgery;the Fourth Military Medical University2;Xi'an 710032;P.R.China |
| Abstract: | To profile the secretory characteristic and cell location of differentially expressed gene GDDR,and explore the biological function of GDDR gene in vitro,EGFP tagged GDDR was respectively expressed in GES-1,HT29 and SGC-7901 cells to confirm the subcellular localization of GDDR.Secretory protein was concentrated from the supernatant and test by western blot.To investigate the biological effect of GDDR on gastric cancer cells,gastric cancer cell line SGC-7901 was transfected with GDDR.EGFP tagged GDDR was re... |
| Keywords: | stomach neoplasm secretory protein cell cycle functional study |
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