| 以赭曲霉毒素A多克隆抗体建立直接竞争酶联免疫吸附分析方法研究 |
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| 引用本文： | 刘运龙.以赭曲霉毒素A多克隆抗体建立直接竞争酶联免疫吸附分析方法研究[J].广西科学,2010,17(3):244-246. |
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| 作者姓名： | 刘运龙 |
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| 作者单位： | 广西粮油科学研究所,广西南宁,530001 |
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| 摘 要： | 将赭曲霉毒素A(OA)抗原免疫大白兔制备多克隆抗体,建立直接竞争酶联免疫吸附分析方法(ELISA)。结果多克隆抗体的效价高达1∶21万。建立的直接竞争ELISA检测方法的下限为0.05ng/ml,线性范围0.05~51.2ng/ml,OA添加到玉米粉中的样品回收率达到94.8%。
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| 关 键 词： | 酶联免疫吸附法 赭曲霉毒素A 多克隆抗体 |
| 收稿时间： | 2009/8/20 0:00:00 |
| 修稿时间： | 2010/4/12 0:00:00 |
Development of Direct Competitive ELISA for Ochratoxin A Assay |
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| LIU Yun-long.Development of Direct Competitive ELISA for Ochratoxin A Assay[J].Guangxi Sciences,2010,17(3):244-246. |
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| Authors: | LIU Yun-long |
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| Institution: | LIU Yun-long(Guangxi Grain and Oil Scientific Institue,Nanning,Guangxi,530001,China) |
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| Abstract: | Ochratoxin A（OA）-KLH conjugates were mixtured with adjuvant and injected into rabbit hypoderm to produce anti-serum.The anti-OA polyclonal antibodies were purified with affinity chromatoghrphic column and used to establishe directe competitive enzyme-linked immunosorbent assay.The results showed that the line arrange of the assay was between 0.05 and 51.2ng/ml.The lowest detectable amount of OA was 0.05ng/ml.OA recovery from corn flour with OA reached to 94.8%. |
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| Keywords: | ELISA ochratoxin A polyclonal antibody |
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