| 黄瓜离体雌核发育的过程及其早期生化变化研究 |
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| 引用本文： | 杜胜利,韩毅科,丛颖,魏爱民,侯锋,陈启民.黄瓜离体雌核发育的过程及其早期生化变化研究[J].南开大学学报,2003,36(2):27-30. |
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| 作者姓名： | 杜胜利 韩毅科 丛颖 魏爱民 侯锋 陈启民 |
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| 作者单位： | [1]天津市黄瓜研究所，天津300192；南开大学生命科学学院，天津300071 [2]天津市黄瓜研究所，天津300192 [3]南开大学生命科学学院，天津300071 |
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| 摘 要： | 本首次研究了黄瓜离体雌核发育的形态及细胞学过程，以及培养早期生理、生化的变化．结果表明：雌核发育在培养的前6d即已开始，24d时形成胚状体，40d时形成成熟的胚状体．雌核发育的前6d，外植体POD酶活性急剧上升，SOD先上升后下降，Q酶活性明显上升但低于非雌核发育，Ca^2 -ATP酶和Mg^2--ATP酶活性变化较小且均低于非雌核发育．雌核发育的外植体IAA和ABA含量明显下降，iPA，Zr，CA3和CA4含量均有所下降并明显低于非雌核发育外植体．
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| 关 键 词： | 黄瓜（cucumis sativus L.） 雌核发育 生化 |
| 文章编号： | 0465-7942(2003)02-0027-04 |
| 修稿时间： | 2002年3月20日 |
HISTOLOGICAL AND EARLY BIOCHEMICAL ASPECTS IN CUCUMBER IN VITRO GYNOGENESIS |
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| DU Shengli ,HAN Yike ,CONG Ying ,WEI Aimin ,HOU Feng ,CHEN Qimin.HISTOLOGICAL AND EARLY BIOCHEMICAL ASPECTS IN CUCUMBER IN VITRO GYNOGENESIS[J].Acta Scientiarum Naturalium University Nankaiensis,2003,36(2):27-30. |
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| Authors: | DU Shengli HAN Yike CONG Ying WEI Aimin HOU Feng CHEN Qimin |
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| Institution: | DU Shengli 1,2,HAN Yike 1,CONG Ying 1,WEI Aimin 1,HOU Feng 1,CHEN Qimin 2 |
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| Abstract: | Histological and early biochemical aspects of cucumber in vitro gynogenesis were studied. The results indicated that cucumber gynogenesis initiated within 6 days of culture. Embryoids were formed at the 24th day, mature embryoids were formed after the 40th day. In the first 6 days of culture, explant POD activity increased dramatically, SOD activity increased at the 3th day and decreased at the 6th day, Q enzyme activity increased obviously but lower than that of non-gynogenetic explant, Ca 2+-ATPase and Mg 2+-ATPase activity changed little and were lower than that of non-gynogenetic explant. IAA and ABA content decreased dramatically and were lower than that of non-gynogenetic explant. Zr, iPA, GA4 and GA3 content changed little and were obviously lower than that of non-gynogenetic explant. |
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| Keywords: | cucumber (cucumis sativus L ) gynogenesis biochemistry |
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