| 南瓜多糖含量测定方法比较 |
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| 引用本文： | 陈立江,王 菁,刘 宇,焉喜臣,王金晶,吴 凡.南瓜多糖含量测定方法比较[J].辽宁大学学报(自然科学版),2014(1):76-81. |
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| 作者姓名： | 陈立江 王 菁 刘 宇 焉喜臣 王金晶 吴 凡 |
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| 作者单位： | 辽宁大学药学院,辽宁沈阳110036 |
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| 摘 要： | 确立一种能够准确测定南瓜多糖含量的方法.采用间接碘量法和苯酚硫酸法分别测定同一批样品南瓜多糖的含量.实验结果表明,间接碘量法测定南瓜多糖的含量平均为43.61%,其中总糖为53.14%,单糖为9.53%.总糖和单糖的平均回收率分别为100.09%和101.51%,相对标准偏差(RSD)为0.63%和0.77%,重现性良好.间接碘量法不仅能测出总糖含量,并且也能对单糖含量有较好评价.该方法准确,简便,稳定性好.
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| 关 键 词： | 南瓜多糖 间接碘量法 苯酚硫酸法 含量测定 |
The Content of Pumpkin Polysaccharide Determination Comparison |
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| CHEN Li-jiang,WANG Jing,LIU Yu,YAN Xi-chen,WANG Jin-jing,WU Fan.The Content of Pumpkin Polysaccharide Determination Comparison[J].Journal of Liaoning University(Natural Sciences Edition),2014(1):76-81. |
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| Authors: | CHEN Li-jiang WANG Jing LIU Yu YAN Xi-chen WANG Jin-jing WU Fan |
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| Institution: | ( College of pharmacy, Liaoning University, Shenyang 110036, China) |
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| Abstract: | A method was developed to determine the contents of pumpkin Polysaccharides accurately. The contents of Pumpkin Polysaccharides in the same batch was determined by indirect iodometric method and Phenol-sulfuric Acid. Experimental results of indirect iodometfic method show that the average content of Pumpkin Polysaccharides is 43.61%, as the total sugar content is 53.14% and the monosaccharide is 9.53%. The average recovery of total sugar and monosaccharide is 100.09% and 101.51% respectively,and the relative standard deviation（RSD） is 0.63% and 0.77% respectively, with good reproducibility. Indirect iodometric method can evaluate the determination of Pumpkin Polysaccharides on total sugar and monosaccharide. This method is accurate, simple and steady. |
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| Keywords: | pumpkin polysaccharides indirect iodometric method Phenol-sulfuric Acid content determination |
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