| 研究生分子生物学综合实验设计──IMPACT蛋白单柱亲和纯化 |
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| 引用本文： | 赵仲麟,李燕,朱伟,张淑利,贾树恒,马斌强,袁超.研究生分子生物学综合实验设计──IMPACT蛋白单柱亲和纯化[J].江西科学,2014(2):130-132,201. |
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| 作者姓名： | 赵仲麟 李燕 朱伟 张淑利 贾树恒 马斌强 袁超 |
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| 作者单位： | [1]河南农业大学理学院,河南郑州450002 [2]郑州轻工业学院食品与生物工程学院,河南郑州450002 [3]河南农业大学农学院,河南郑州450002 |
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| 基金项目： | 国家自然科学基金（31100067）. |
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| 摘 要： | 设计了一个研究生分子生物学综合实验,利用内含肽介导的IMPACT系统对蛋白质进行纯化。将目的基因构建入pTWIN2载体,转化至大肠杆菌ER 2566中诱导其表达,进化亲和层析,检测其表达情况。该方法具备比以往亲和层析纯化方法更加经济的特点,可提高目的蛋白表达的成功率。通过本实验使学生掌握的分子生物学研究中最新的蛋白质分离纯化操作技术,为进一步学习和掌握复杂、综合性的分子生物学技术打下扎实的基础。
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| 关 键 词： | 分子生物学 研究生实验 内含肽 蛋白纯化 |
A Postgraduate Experimental Design of Molecular Biology -IMPACT Single Column System for Protein Affinity Purification |
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| ZHAO Zhong-lin,LI Yan,ZHU Wei,ZHANG Shu-li,JIA Shu-heng,MA Bin-qiang,YUAN Chao.A Postgraduate Experimental Design of Molecular Biology -IMPACT Single Column System for Protein Affinity Purification[J].Jiangxi Science,2014(2):130-132,201. |
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| Authors: | ZHAO Zhong-lin LI Yan ZHU Wei ZHANG Shu-li JIA Shu-heng MA Bin-qiang YUAN Chao |
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| Institution: | . ( 1. College of Sciences, Henan Agricultural University, Henan Zhengzhou 450002 PRC ; 2. School of Food and Biological Engineering, Zhengzhou University of Light Industry, Henan Zhengzhou 450002 PRC ; 3. College of Agronomy, Henan Agricultural University, Henan Zhengzhou 450002 PRC) |
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| Abstract: | A postgraduate experiment of molecular biology was designed to express target protein in E. coli by IMPCT protein purification system. The target gene was inserted into vector pTWIN2, and then transformed to E. coli ER2566 for protein expression. This affinity purification system was a rap- id and efficient method, which could improve the solubility of target protein. By this experiment, the postgraduate students could learn the modern novel manipulation techniques of molecular biology. It would build a solid foundation for their future research. |
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| Keywords: | Molecular biology Postgraduate experiment Intein Protein purification |
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