| Cloning of rainbow trout (Oncorhynchus mykiss ) histone H3 promoter and the activity analysis in rare minnow (Gobiocypris rarus) |
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| 作者姓名： | MAO Weifeng SUN Yonghu WANG Yaping WU Gang CHEN Shangping ZHU Zuoyan |
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| 作者单位： | State Key Laboratory of Freshwater Ecology and Biotechnology,Institute of Hydrobiology,Chinese Academy of Sciences,Wuhan 430072,China,State Key Laboratory of Freshwater Ecology and Biotechnology,Institute of Hydrobiology,Chinese Academy of Sciences,Wuhan 430072,China,State Key Laboratory of Freshwater Ecology and Biotechnology,Institute of Hydrobiology,Chinese Academy of Sciences,Wuhan 430072,China,State Key Laboratory of Freshwater Ecology and Biotechnology,Institute of Hydrobiology,Chinese Academy of Sciences,Wuhan 430072,China,State Key Laboratory of Freshwater Ecology and Biotechnology,Institute of Hydrobiology,Chinese Academy of Sciences,Wuhan 430072,China,State Key Laboratory of Freshwater Ecology and Biotechnology,Institute of Hydrobiology,Chinese Academy of Sciences,Wuhan 430072,China |
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| 基金项目： | 国家重点基础研究发展计划(973计划) |
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| 摘 要： | Rainbow trout histone H3(RH3)promoter was cloned via high fidelity PCR.The cloned RH3 promoter was inserted into a promoter-lacked vector pEGFP-1,resulting in an expression vector pRH3EGFP-1.The linearized pRH3EGFP-1 was microinjected into fertilized eggs of rare minnows and the sequential embryogenetic processes were monitored under a fluorescent microscope.Strong green fluorescence was ubiquitously observed at as early as the gastrula stage and then in various tissues at the fry stage.The results indi cate that RH3 promoter,as a piscine promoter,could serve in producing transgenic Cyprinoid such as rare minnow.Promoter activity of RH3,CMV and common carp β-actin(CA)were compared in rare minnow by the expression of respective recombinant EGFP vectors.The expression of pCMVEGFP occurred earlier than the following one,pRH3EGFP-1,and then pCAEGFP during the embryogenesis of the transgenics.Their expression activities demonstrated that the CMV promoter is the strongest one,followed by the CA and then the RH3.
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| 关 键 词： | enhanced green fluorescent protein(EGFP) histone H3 promoter transgenic fish rare minnow |
Cloning of rainbow trout (Oncorhynchus mykiss ) histone H3 promoter and the activity analysis in rare minnow (Gobiocypris rarus) |
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| MAO Weifeng,SUN Yonghu,WANG Yaping,WU Gang,CHEN Shangping,ZHU Zuoyan.Cloning of rainbow trout (Oncorhynchus mykiss ) histone H3 promoter and the activity analysis in rare minnow (Gobiocypris rarus)[J].Progress in Natural Science,2004,14(4):322-326. |
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| Authors: | Mao Weifeng SUN Yonghua WANG Yaping WU Gang CHEN Shangping ZHU Zuoyan |
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| Institution: | State Key Laboratory of Freshwater Ecology and Biotechnology,Institute of Hydrobiology,Chinese Academy of Sciences,Wuhan 430072,China |
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| Abstract: | Rainbow trout histone H3(RH3)promoter was cloned via high fidelity PCR.The cloned RH3 promoter was inserted into a promoter-lacked vector pEGFP-1,resulting in an expression vector pRH3EGFP-1.The linearized pRH3EGFP-1 was microinjected into fertilized eggs of rare minnows and the sequential embryogenetic processes were monitored under a fluorescent microscope.Strong green fluorescence was ubiquitously observed at as early as the gastrula stage and then in various tissues at the fry stage.The results indi cate that RH3 promoter,as a piscine promoter,could serve in producing transgenic Cyprinoid such as rare minnow.Promoter activity of RH3,CMV and common carp β-actin(CA)were compared in rare minnow by the expression of respective recombinant EGFP vectors.The expression of pCMVEGFP occurred earlier than the following one,pRH3EGFP-1,and then pCAEGFP during the embryogenesis of the transgenics.Their expression activities demonstrated that the CMV promoter is the strongest one,followed by the CA and then the RH3. |
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| Keywords: | enhanced green fluorescent protein(EGFP) histone H3 promoter transgenic fish rare minnow |
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