| 蛇足石杉内生菌的分离与鉴定 |
| |
| 引用本文： | 张琳,段紫英,耿欣,李克刚,魏宗,胡颂平. 蛇足石杉内生菌的分离与鉴定[J]. 吉首大学学报(自然科学版), 2010, 31(5): 79-84 |
| |
| 作者姓名： | 张琳 段紫英 耿欣 李克刚 魏宗 胡颂平 |
| |
| 作者单位： | （1.江西农业大学理学院,江西 南昌 330045；2.吉首大学资环学院,湖南 吉首 416000；3.江西农业大学生物工程学院，江西 南昌 330045；4.南昌市微生物学重点实验室，江西 南昌 330045） |
| |
| 基金项目： | 上海市农业生物基因中心重点项目，湖南省科技计划项目，江西农业大学人事基金项目 |
| |
| 摘 要： | 从蛇足石杉的培养组织中分离内生真菌，利用传统方法，观察菌落的外观形态，对照《真菌鉴定手册》对其进行鉴定.通过马铃薯培养基（PDA）培养菌体，通过三点接种、分区划线的方法进行分离纯化，利用显微摄影的技术进行形态观察和鉴定.从蛇足石杉的茎尖培养组织中分离出7株内生真菌，经鉴定它们分别属于盘菌属、镶孢霉属、瓶梗青霉属、青霉属、沙卡氏属、曲霉属.
|
| 关 键 词： | 蛇足石杉 内生菌 分离 鉴定 |
Isolation and Identification of Endophytic Fungi of Huperzia Serrata |
| |
| ZHANG Lin,DUAN Zi-ying,GENG Xin,LI Ke-gang,WEI Zong,HU Song-ping. Isolation and Identification of Endophytic Fungi of Huperzia Serrata[J]. Journal of Jishou University(Natural Science Edition), 2010, 31(5): 79-84 |
| |
| Authors: | ZHANG Lin DUAN Zi-ying GENG Xin LI Ke-gang WEI Zong HU Song-ping |
| |
| Affiliation: | (1.College of Science,Jiangxi Agricultural University,Nanchang 330045,China;2.College of Biological Resource and Environment Science,Jishou University,Jishou 416000,Hunan China;3.College of Bioscience and Engineering,Jiangxi Agricultural University,Nanchang 330045,China;4.Key Laboratory of Microbiology in Nanchang City,Nanchang 330045,China) |
| |
| Abstract: | Endophytic fungi of the cultured tissue from Huperzia serrata were isolated and identified by observing the appearance shape of colonies according to the Identification Handbook of Fungus.In this study,the endophytic fungi were cultured in PDA medium and purified by the methods of agar plate.At the same time the shape of these fungi was observed and identified by microphotograph technology.The results showed that there were seven endophytic fungi species isolated from the cultured tissue of the tip stem of Huperzia serrata,which respectively belonged to Peziza Dill.ex Fr,Paecilomyces Bainier,Saccardia Cooke,A.ustus,P.chrysogenim,Coniothecium Cda. |
| |
| Keywords: | huperzia serratta（thunb）trev. endophytic fungi isolation identification |
| 本文献已被 维普 万方数据 等数据库收录！ |
| 点击此处可从《吉首大学学报(自然科学版)》浏览原始摘要信息 |
|
点击此处可从《吉首大学学报(自然科学版)》下载全文 |
|
