Marinamide, a novel alkaloid and its methyl ester produced by the application of mixed fermentation technique to two mangrove endophytic fungi from the South China Sea

For a long time, terrestrial microorganisms are abundant natural pharmaceutical resource for human, but it becomes more and more difficult to find new species and microorganisms with special functions from land as the research goes on. Consequently, the s…

Marinamide, a novel alkaloid and its methyl ester produced by the application of mixed fermentation technique to two mangrove endophytic fungi from the South China Sea

A novel 1-isoquinolone analog desig- nated as marinamide (A) and its methyl ester (B), were produced by the application of mixed fermenta- tion technique to two mangrove endophytic fungi (strains Nos. 1924 and 3893) from the South China Sea. Their structures were elucidated by compre- hensive spectra methods (mainly by 2D NMR) as 4- (2-pyrrolyl)-1-isoquinolone-3-carboxylic acid (A) and methyl 4-(2-pyrrolyl)-1-isoquinolone-3-carboxylate (B), respectively. Compounds A and B were not obtained when either strain was cultured individually under the same conditions. The results showed that the appli- cation of mixed fermentation technique maybe rep- resents a potentially important approach to discover novel metabolites.