三峡区域特色药材宜昌润楠正丁醇提取物体外抗炎活性研究

目的：探讨宜昌润楠正丁醇提取物（MCB）的体外抗炎活性．方法：利用细菌脂多糖（LPS）刺激经佛波酯（PMA）诱导的人单核细胞THP-1建立体外炎症模型，MTT法检测样品的细胞毒性，ELISA方法检测药物干预前后细胞上清液中IL-1β、IL-6、TNF—α和NO的分泌量，综合评价MCB对炎性细胞因子和介质的抑制作用．结果：MCB显著性地抑制了IL-6和TNF—a以及NO的产生，并且其抑制作用具有时间和剂量依耐性，但对IL-1β没有明显的抑制效果．结论：本研究初步验证了宜昌润楠的体外抗炎作用，为其民间应用提供了理论依据，也为进-步分离生物活性物质提供了方向．

Anti-inflammatory Activity in Vitro of n-BuOH Extracts from Machilus Ichangensis

To evaluate the anti-inflammatory activity in vitro of n-BuOH extracts from Machilus ichangensis （MCB）. Inflammation model in vitro was established by stimulating human acute monocytic leukemia cell line （THP-1） with lipopolysaccharide （LPS） after which were differentiated into macrophages by phorbol ester （PMA） ; Cytotoxicity of MCB was determined by MTT assay; The anti-inflammatory potential of MCB was evaluated by monitoring its effects on the modulation of cytokines and the mediators of inflammation, such as interleukin 1β（IL-1β）, interleukin 6 （IL-6）, tumor necrosis factor-α （TNF-α） and nitric oxide （NO） in cell culture medium using ELISA methods. MCB showed great significant dose- and time-dependent inhibitory ac- tivity on IL-6, TNF-α and NO synthesis, but slight effect on IL-1β, all of which are involved in the inflamma- tory response. This study preliminary validate the anti-inflammatory activity in vitro of MCB and for its tradi- tional applications provide the pharmacological basis. Therefore, this plant is a promising candidate for fur- ther screening of its active compounds through activity-guided fractionation.