| 鹿茸水提取物及其蛋白酶解物对小鼠脾淋巴细胞增殖的影响 |
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| 引用本文： | 赵磊,王成涛,籍保平. 鹿茸水提取物及其蛋白酶解物对小鼠脾淋巴细胞增殖的影响[J]. 北京工商大学学报(自然科学版), 2011, 29(3): 20-27 |
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| 作者姓名： | 赵磊 王成涛 籍保平 |
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| 作者单位： | 1. 北京工商大学食品学院/食品添加剂与配料北京高校工程研究中心,北京,100048
2. 中国农业大学食品科学与营养工程学院,北京,100083 |
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| 摘 要： | 研究了鹿茸水提取物中非蛋白成分、粗蛋白酶解物及分离组分对小鼠脾淋巴细胞增殖的影响.非蛋白成分和酶解物的分离分别采用HPLC法和超滤法.应用WST-8法检测各分离组分对小鼠脾淋巴细胞增殖的影响,采用高分辨质谱对非蛋白成分进行初步鉴定.结果显示,非蛋白成分对未经刀豆蛋白A(ConA)诱导的小鼠脾细胞增殖的促进作用优于其分离组分,Fr.Ⅲ和Ⅳ对ConA诱导的脾淋巴细胞增殖有抑制作用,初步鉴定其为碱基和核苷酸的混合物.分子量大于10 kDa的肽段,单独可刺激小鼠脾细胞增殖,且对ConA诱导的T淋巴细胞增殖有抑制作用.肽段分子量大小对其免疫调节活性影响较大,但并未呈现直接的相关性.肽的氨基酸组成、序列及构型等也是影响其活性的重要因素.
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| 关 键 词： | 鹿茸水提取物 非蛋白成分 粗蛋白 酶解物 小鼠脾淋巴细胞 |
Effect of Aqueous Extract of Velvet Antler and Its Crude Protein Hydrolysates on Murine Splenocytes Proliferation |
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| ZHAO Lei,WANG Cheng-tao and JI Bao-ping. Effect of Aqueous Extract of Velvet Antler and Its Crude Protein Hydrolysates on Murine Splenocytes Proliferation[J]. Journal of Beijing Technology and Business University:Natural Science Edition, 2011, 29(3): 20-27 |
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| Authors: | ZHAO Lei WANG Cheng-tao JI Bao-ping |
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| Affiliation: | ZHAO Lei1,WANG Cheng-tao1,JI Bao-ping2(1.School of Food/Beijing Higher Institution Engineering Research Center of Food Additives and Ingredients,Beijing Technology and Business University,Beijing 100048,China,2.College of Food Science and Nutritional Engineering,China Agricultural University,Beijing 100083,China) |
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| Abstract: | The aim of the present study was to investigate the effect of non-protein components(AEVA-S),crude protein hydrolysates and their fractions from aqueous extract from velvet antler(AEVA) on murine splenocytes proliferation.AEVA-S and crude protein hydrolysates were fractionated by HPLC and ultrafiltration,respectively.The effect of each fraction on the proliferation of murine splenocytes was detected by WST-8.The non-protein components were preliminary identified by high resolution mass spectrometry.The res... |
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| Keywords: | aqueous extract of velvet antler non-protein components crude protein enzymatic hydrolysates murine splenocyte |
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