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| 酒醅微生物DNA提取预处理方法研究 | |
| 引用本文： | 沈才萍,李德林,沈才洪,邓波,沈小娟,敖宗华.酒醅微生物DNA提取预处理方法研究[J].四川理工学院学报(自然科学版),2013,26(3):16-20. |
| 作者姓名： | 沈才萍 李德林 沈才洪 邓波 沈小娟 敖宗华 |
| 作者单位： | 1. 泸州老窖股份有限公司,四川泸州,646000 2. 四川理工学院生物工程学院,四川自贡,643000 3. 泸州老窖股份有限公司,四川泸州646000;国家固态酿造工程技术研究中心,四川泸州646000 4. 泸州老窖股份有限公司,四川泸州646000;四川理工学院生物工程学院,四川自贡643000;国家固态酿造工程技术研究中心,四川泸州646000 |
| 基金项目： | 四川省科技支撑计划项目 |
| 摘 要： | 浓香型白酒的酒醅中含有大量的单糖、寡糖、多糖、色素、有机酸和腐殖酸等有机杂质,严重影响对酒醅中微生物进行相关分子生物学研究。如何通过预处理方法降低杂质的影响,快速成功提取高质量的DNA直接影响整个分子生物学实验的准确性。实验采用无菌水、TENP缓冲液、PBS缓冲液分别对酒醅样品进行预处理。通过对样品浓度、纯度、DGGE丰度等指标的比较,确定了PBS预处理法为最佳预处理方法。 |
| 关 键 词： | 浓香型白酒 酒醅 预处理 |
Pretreatment Method Study of DNA Extraction from Fermented Grains Microbial |
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| SHEN Cai-ping , LI De-lin , SHEN Cai-hong , DENG Bo , SHEN Xiao-juan , AO Zong-hua.Pretreatment Method Study of DNA Extraction from Fermented Grains Microbial[J].Journal of Sichuan University of Science & Engineering:Natural Science Editton,2013,26(3):16-20. | |
| Authors: | SHEN Cai-ping LI De-lin SHEN Cai-hong DENG Bo SHEN Xiao-juan AO Zong-hua |
| Institution: | 1,2,3(1.Luzhou Laojiao Co.,Ltd.,Luzhou 646000,China;2.School of Biotechnology Engineering, Sichuan University of Science & Engineering,Zigong 643000,China;3.National Solid Brewing Engineering Technology Research Center,Luzhou 646000,China) |
| Abstract: | The fermented grains of Luzhou-flavor liquor contain a lot of organic impurities,such as monosaccharide,oligosaccharide,polysaccharide,pigment,organic acid,humic acid,etc.These impurities seriously affect molecular biology research on the microorganism in fermented grains.How to reduce the impurities influence,and to quickly and successfully extract DNA of high quality through the pretreatment method,will directly affect the accuracy of the whole experiment.In order to solve this problem,this research use Sterile water,TENP buffer and PBS buffer,to deal with the samples.Through the index comparison of sample concentration,purity,DGGE abundance and so on,PBS method is determined to be the best pretreatment method. |
| Keywords: | luzhou-flavor liquor fermented grains pretreatment |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |