浙江产蝮蛇(Agkistrodon halys Pallas)蛇毒的柱层析分离及磷酸二酯酶的大规模纯化 |
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引用本文: | 涂光俦. 浙江产蝮蛇(Agkistrodon halys Pallas)蛇毒的柱层析分离及磷酸二酯酶的大规模纯化[J]. 科学通报, 1979, 24(13): 617-617 |
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作者姓名: | 涂光俦 |
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作者单位: | 中国科学院昆明动物研究所(涂光俦,冉永禄,武天爱),中国科学院昆明动物研究所(于力) |
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摘 要: | 蛇毒磷酸二酯酶是核酸结构分析的一种重要工具酶。自从1932年Uzawa首先证实蛇毒中广泛存在磷酸二酯酶以来,关于该酶的分离纯化研究,Laskowski等实验室做了许多工作,英国B.D.H.公司,美国Sigma和Worthington公司已有蛇毒磷酸二酯酶的商品制剂出售。 我国具有丰富的蛇毒资源,而且这些蛇毒都含有磷酸二酯酶。我们在浙江产蝮蛇毒柱
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收稿时间: | 1978-11-18 |
CHROMATOGRAPHY FRACTIONATION OF THE VENOM OF AGKISTRODON HALYS PALLAS AND THE PURIFICATION OF THE PHOSPHODIESTERASE |
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Affiliation: | TU GUANG-CHOU RAN YONG-LU WU TIAN-AI YU LI |
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Abstract: | The venom of Agkistrodon halys Pallas (from Zhejiang Province) has been fractionated on DEAE-Sephadex A-50 into 14 different components. A simple and economic method for purifying the phosphodiesterase on a large scale is described. Compared with the same kind of products from other countries, satisfactory results have been achieved in the analysis of ribose and deoxyribose oligo-nucleotides with this enzyme. |
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