Proliferation and cell loss of human leukemic cell subpopulations in liquid culture |
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| Authors: | P A Bernabei F C Agostino R Bezzini R Saccardi V Gattei V Santini M Casini P Rossi Ferrini |
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| Institution: | Div. Ematologia, Università degli Studi, Policlinico di Careggi, Firenze (Italy). |
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| Abstract: | A kinetic study was performed on leukemic blasts from patients with acute myeloid leukemia, separated into 2 subpopulations by a specific density gradient. The growth curve and the 3H]-thymidine uptake were simultaneously analyzed. While cumulative nucleotide uptake fitted with the growth kinetics in the low-density fraction, such a concordance was not found in the high-density subpopulation. That indicated the occurrence of simultaneous growth and loss in the high density fraction, which could not be evaluated by a simple numerical determination. |
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