莠去津对雌性日本沼虾的毒性作用

研究莠去津对雌性日本沼虾的生殖毒性,为其养殖水体水质管理提供依据.以概率作图法计算日本沼虾24,48,72和96 h的半致死质量浓度,并观察各暴露组卵巢组织结构的变化.通过胁迫实验,研究莠去津对暴露4 d和8 d的日本沼虾卵巢乳酸脱氢酶(LDH)比活力的影响.结果表明:22℃时,莠去津对雌性日本沼虾24,48,72和96 h的半致死质量浓度分别为134.23,90.24,52.57和46.36 mg/L,安全质量浓度为4.64 mg/L;卵巢急性毒性实验切片显示,随莠去津质量浓度的增大,卵母细胞间隙增大,胞体膨大、变形.胁迫实验表明,低质量浓度莠去津在短时间内对卵巢LDH活力有诱导作用,当质量浓度高于0.46 mg/L时,LDH活力明显受到抑制.本研究提示,莠去津对雌性日本沼虾具有生殖毒性作用.

Toxic Effect of Atrazine on Female Macrobrachium nipponense

Reproductive toxic effects of atrazine on female Macrobrachium nipponense were studied to provide evidence for the water quality management of aquaculture.Semi-lethal mass concentrations of atrazine to M.nipponense at 24,48,72 and 96 h were calculated by probability mapping.The changes of ovarian tissue structure in each exposure group were observed.Ovarian lactate dehydrogenase(LDH) specific activity at the atrazine exposure of 4 days and 8 days was detected by stress experiment.The results showed that the semi-lethal mass concentration of atrazine to M.nipponense at 24,48,72 and 96 h at 22 ℃ were 134.23,90.24,52.57 and 46.36 mg/L,respectively,and the safe mass concentration was 4.64 mg/L.The ovarian sections in acute toxicity test showed that the intercellular space of oocytes increased and the cell bodies enlarged and deformed with the increase of the mass concentration of atrazine.The stress experiment demonstrated that atrazine at a low mass concentration increased ovarian LDH specific activity in a short time.However,atrazine at the mass concentration above 0.46 mg/L inhibited the ovarian LDH specific activity obviously.The study indicated that atrazine had the reproductive toxic effects on female M.nipponense.