| 产环己酰亚胺新菌株YIM41004种子培养基优化研究初报 |
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| 引用本文： | 陈义光,刘祝祥,李铭刚,文孟良,郭端强,徐丽华.产环己酰亚胺新菌株YIM41004种子培养基优化研究初报[J].云南大学学报(自然科学版),2006,28(2):173-177. |
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| 作者姓名： | 陈义光 刘祝祥 李铭刚 文孟良 郭端强 徐丽华 |
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| 作者单位： | 1. 云南大学,云南省微生物研究所,教育部微生物资源重点实验室,云南,昆明,650091;吉首大学,生物资源与环境科学学院,湖南,吉首,416000
2. 云南大学,云南省微生物研究所,教育部微生物资源重点实验室,云南,昆明,650091 |
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| 基金项目： | 国家自然科学基金资助项目(30270004)，云南省烟草公司基金资助项目(04A16)，云南省自然科学基金重点资助项目(2004C0002Z) |
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| 摘 要： | 对产环己酰亚胺新菌株Streptomyces yunnanensis YIM41004的摇瓶种子培养基进行了优化,获得最佳配方为葡萄糖25 g,蛋白胨7.5 g,酵母膏7.5 g,CaCO33 g,MgSO4.7H2O 1 g,MnSO4.4H2O 0.1 g,KH2PO40.2 g,水1 000 mL;最佳培养条件为起始pH7.8,500mL种子瓶装量100mL,最佳种龄32~36 h.用优化后的种子培养基培养种子用于摇瓶发酵,结果表明在接种量为10%体积分数时,环己酰亚胺产量达到最高,为498.9 mg.L-1,比用原始种子培养基时的产量450 mg.L-1高出10.7%.
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| 关 键 词： | Streptomyces yunnanensis YIM41004 环己酰亚胺 种子培养基 优化 |
| 文章编号： | 0258-7971(2006)02-0173-05 |
| 修稿时间： | 2005年10月5日 |
Preliminary study on the optimization of seed culture medium for cycloheximide-producing strain YIM41004 |
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| CHEN Yi-guang,LIU Zhu-xiang,LI Ming-gang,WEN Meng-liang,GUO Duan-qiang,XU Li-hua.Preliminary study on the optimization of seed culture medium for cycloheximide-producing strain YIM41004[J].Journal of Yunnan University(Natural Sciences),2006,28(2):173-177. |
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| Authors: | CHEN Yi-guang LIU Zhu-xiang LI Ming-gang WEN Meng-liang GUO Duan-qiang XU Li-hua |
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| Institution: | 1. Key Laboratory for Microbial Resources of Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, China;2. College of Biological Resource & Environment Science, Jishou University, Jishou 416000, China |
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| Abstract: | The composition of seed culture medium and the culture conditions for cycloheximide-producing new strain Streptomyces yunnanensis YIM41004 were optimized.The optimum composition was as following:glucose 25g,peptone 7.5g,yeast extract 7.5g,CaCO3 3g,MgSO4·7H2O 1g,MnSO4·4H2O 0.1g,KH2PO4 0.2g,distilled water 1000mL.The optimal pH value was 7.8,the broth volume in 500mL flask was 100mL,and the optimal seed age was 32-36h.Inoculated with 10%(V/V) seed broth and cultured with the optimized seed culture medium,the cycloheximide production level of flask ferrmentation reached up to 498.9(mg·L-1),which was 10.7%higher than that cultured with the original seed broth culture. |
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| Keywords: | Streptomyces yunnanensis YIM41004 cycloheximide seed culture medium optimization |
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