Expression of matrix metalloproteinase-28 in human normal cytotrophoblast cells and a choriocarcinoma cell line,JEG-3 |
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Authors: | Zhang?Jian Cao?Yujing Liu?Weimin Zhao?Bingshun Zeng?Guoqing Email author" target="_blank">Duan?Enkui?Email author |
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Institution: | (1) State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, 100080 Beijing, China |
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Abstract: | There are striking similiarities present between the behavior of invasive placental cells and that of invasive cancer cells. Matrix metalioproteinases (MMPs) are one of the most important mediators. MMP-28, the new member ofMMPs, was sequenced and identified recently. Expression of MMP-28 mRNA and protein in the cytotrophoblast cells anda choriocarcinoma cell line, JEG-3 cell, was conducted by zymography, RT-PCR and Northern blot. There is MMP-28mRNA expression in both the cytotrophoblast cells and JEG-3 cells by RT-PCR. The activity of MMP-28 in cytotrophoblast cells was significantly weaker than that in JEG-3 (P < 0.01) by zymography. Furthermore, mRNA expression of MMP-28 was significantly stronger (P < 0.001) in JEG-3than in human cytotrophoblast cells in a time-dependent way by Northern Blot. Our results suggest that MMP-28 may play a role in some of the tissue-remodeling events associated with normal pregnancy and tumor progression.`` |
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Keywords: | cytotrophoblast cell choriocarcinoma cell line JEG-3 matrix metaHoproteinases MMP-28 |
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