| 反相高效液相色谱法快速测定谷氨酰胺发酵过程中主要游离氨基酸 |
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| 引用本文： | 陈旭,雍克岚. 反相高效液相色谱法快速测定谷氨酰胺发酵过程中主要游离氨基酸[J]. 上海大学学报(自然科学版), 2000, 6(6): 530-532 |
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| 作者姓名： | 陈旭 雍克岚 |
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| 作者单位： | 上海大学,生命科学学院,上海,200436 |
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| 摘 要： | 谷氨酸胺发酵过程代谢的主要游离氨基酸是谷氨酸胺和谷氨酸．用反相高效液相色谱技术，采用柱前衍生、荧光检测的方法可快速测定其含量．本法衍生步骤简单、灵敏度高、选择性强．利用该技术对正常谷氨酸胺发酵过程代谢的两种主要氨基酸含量变化进行了分析，为谷氨酸胺发酵过程分析提供了可靠的依据．
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| 关 键 词： | 发酵 过程分析 高效液相色谱法 谷氨酸胺 谷氨酸 |
| 修稿时间： | 2000-08-28 |
Determination of Free Amino Acids in Glutamine Fermentation Broth by Reversed-phase High Performance Liquid Chromatography |
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| CHEN Xu,YONG Ke-fan. Determination of Free Amino Acids in Glutamine Fermentation Broth by Reversed-phase High Performance Liquid Chromatography[J]. Journal of Shanghai University(Natural Science), 2000, 6(6): 530-532 |
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| Authors: | CHEN Xu YONG Ke-fan |
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| Abstract: | Main free amino acids are glutamine and glutamic in glutamine fermentation process. By reversed-phase high performance liquid chromatography (RP-HPLC) they can be detected rapidly with precolumn derivatization and fluorescence detector. The method is simple, sensitive and of high selectivity. With this method we analyze the variation in the contents of two free amino acids content in normal Gin fermentation process. It provides reliable basis for Gin fermentation process analysis. |
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| Keywords: | fermentation process analysis high performance liquid chromatography glutamine glutamic acid |
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