| RNAi-mediated knockingdown of rlpk2 gene retarded soybean leaf senescence |
| |
| 作者姓名： | LIXiaoping MAYuanyuan LIPengli ZHANGLiwen WANGYong ZHANGRen WANGNingning |
| |
| 作者单位： | [1]DepartmentofPlantBiologyandEcology，NankaiUniversity，Tianjin300071,China [2]DepartmentofPlantBiologyandEcology，NankaiUniversity，Tianjin300071,China／／DepartmentofBiologicalSciences，University,ofWollongong，NSW2522,Australia |
| |
| 摘 要： | Leaf senescence that occurs in the last stage of leaf development is a genetically programmed process. It is very significant to elucidate the molecular mechanisms that control the initiation and progression of leaf senescence and the way the senescence signal is transduced. In a previous study on artificially induced soybean leaf senescence, we cloned a novel gene designated rlpk2 (Genbank Accession No. AY687391) that encodes a leucine-rich repeat (LRR) receptor like protein kinase. The expression level of rlpk2 gene was shown to be strongly up-regulated during both the natural leaf senescence process in this report and the artificially induced primary-leaf-senescence process in our previous work. The RNA interference (RNAi)-mediated knocking-down of rlpk2 dramatically retarded both the natural and nutrient deficiency-induced leaf senescence in transgenic soybean. The transgenic leaves showed more cell-aggregated surface structure and higher content of chlorophyll.
|
| 关 键 词： | 大豆 RNA干涉 遗传因子 数叶 |
| 收稿时间： | 2005-02-07 |
RNAi-mediated knocking-down ofrlpk2 gene retarded soybean leaf senescence |
| |
| LIXiaoping MAYuanyuan LIPengli ZHANGLiwen WANGYong ZHANGRen WANGNingning.RNAi-mediated knockingdown of rlpk2 gene retarded soybean leaf senescence[J].Chinese Science Bulletin,2005,50(12):1218-1224. |
| |
| Authors: | Xiaoping Li Yuanyuan Ma Pengli Li Liwen Zhang Yong Wang Ren Zhang Ningning Wang |
| |
| Institution: | LI Xiaoping1, MA Yuanyuan1, LI Pengli1, ZHANG Liwen1, WANG Yong1, ZHANG Ren1,2 & WANG Ningning1 1. Department of Plant Biology and Ecology, Nankai University, Tianjin 300071, China; 2. Department of Biological Sciences, University of Wollongong, NSW2522, Australia |
| |
| Abstract: | Leaf senescence that occurs in the last stage of leaf development is a genetically programmed process. It is very significant to elucidate the molecular mechanisms that control the initiation and progression of leaf senescence and the way the senescence signal is transduced. In a previous study on artificially induced soybean leaf senescence, we cloned a novel gene designated rlpk2 (Genbank Accession No. AY687391) that encodes a leucine-rich repeat (LRR) receptor like protein kinase. The expression level of rlpk2 gene was shown to be strongly up-regulated during both the natural leaf senescence process in this report and the artificially induced primary-leaf-senescence process in our previous work. The RNA interference (RNAi)-mediated knocking-down of rlpk2 dramatically retarded both the natural and nutrient deficiency-induced leaf senescence in transgenic soybean. The transgenic leaves showed more cell-aggregated surface structure and higher content of chlorophyll. |
| |
| Keywords: | soybean leaf senescence RNA interference gene knockdown |
| 本文献已被 CNKI 维普 万方数据 SpringerLink 等数据库收录！ |
| 点击此处可从《中国科学通报(英文版)》浏览原始摘要信息 |
| 点击此处可从《中国科学通报(英文版)》下载免费的PDF全文 |
