| 不知火杂柑叶片黄化机理初探 |
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| 引用本文： | 肖家欣,胡娟,陈世林,周守标,张绍铃. 不知火杂柑叶片黄化机理初探[J]. 安徽师范大学学报(自然科学版), 2010, 33(2): 153-158. DOI: 10.3969/j.issn.1001-2443.2010.02.012 |
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| 作者姓名： | 肖家欣 胡娟 陈世林 周守标 张绍铃 |
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| 作者单位： | 安徽师范大学生命科学学院,重要生物资源保护与利用研究安徽省重点实验室,芜湖,241000;南京农业大学园艺学院,南京,210095;安徽师范大学生命科学学院,重要生物资源保护与利用研究安徽省重点实验室,芜湖,241000;湖北省宜昌市夷陵区农业局,宜昌,443100;南京农业大学园艺学院,南京,210095 |
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| 基金项目： | 安徽省自然科学基金,China Postdoctoral Science Foundation funded project,The foundation of Provincial Key Lab of Biotic Environment and Ecological Safety in Anhui |
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| 摘 要： | 应用电感耦合等离子发射光谱仪、H-600型透射电子显微镜研究了不知火杂柑叶片异常黄化及其对解剖特征的影响.主要结果表明:不知火黄化叶的Zn、Mg和Ca含量均处于低量水平,且均显著低于蒰柑;而其SOD、POD和CAT活性、MDA含量均相对高于其正常叶.不知火黄化叶叶肉细胞的细胞核核膜模糊降解,叶绿体数目明显减少,一些叶绿体形态发生肿胀、呈球形,类囊体片层结构模糊,叶绿体基质中质体小球数量显著增多,细胞质中出现了多泡体.本文还就不知火叶片异常黄化的原因及其对解剖特征的影响进行了讨论.
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| 关 键 词： | 不知火杂柑 叶片黄化 矿质元素含量 生理指标 解剖及超微结构 |
Preliminary Study on the Mechanism of Leaf Chlorosis of Shiranuhi |
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| XIAO Jia-xin,HU Juan,CHEN Shi-lin,ZHOU Shou-biao,ZHANG Shao-ling. Preliminary Study on the Mechanism of Leaf Chlorosis of Shiranuhi[J]. Journal of Anhui Normal University(Natural Science Edition), 2010, 33(2): 153-158. DOI: 10.3969/j.issn.1001-2443.2010.02.012 |
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| Authors: | XIAO Jia-xin HU Juan CHEN Shi-lin ZHOU Shou-biao ZHANG Shao-ling |
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| Affiliation: | XIAOJia-xin1,3,HUJuan1,CHENShi-lin2,ZHOUShou-biao1,ZHANG Shao-ling3(1.Provincial Key Lab of the Conservation and Exploitation of Biological Resources,College of Life Sciences,Anhui Normal University,Wuhu 241000,China,2.Bureau of Agriculture of Yiling Region,Yichang 443100,3.College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China.) |
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| Abstract: | Leaf abnormal chlorosis of shiranuhi(Citrus reticulata ×(C. reticulata×C. sinenesis)) and its effects on anatomical features were investigated with inductively coupled plasma optical emission spectrometry and H-600 transmission electron microscope. The main results showed that Zn, Mg and Ca concentrations in chlorotic leaves from shiranuhi were at low levels and significantly lower than those of ponkan(C. reticulata), while superoxide dismutase, peroxidase and catalase activities, malondialdehyde contents were relatively high in chlorotic leaves compared with its normal leaves. In mesophyll cells of chlorotic leaves from shiranuhi, the number of chloroplast significantly decreased, nuclear envelope became blurred, some chloroplasts swelled and their shapes turned to round, thylakoid lamellar structures were vague, and large numbers of plastoglobuli were found in stroma of chloroplast, several multivesicular bodies were additional localized in cytoplasm. Causes of leaf abnormal chlorosis of shiranuhi and its effects on anatomical features were discussed herein. |
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| Keywords: | shiranuhi leaf chlorosis mineral element content physiological index anatomical and ultrastucture |
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