| 乙二胺羟丙基壳聚糖固定化天门冬酰胺酶的反应性质 |
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| 引用本文： | 方波,霍红光,宋道云,江体乾. 乙二胺羟丙基壳聚糖固定化天门冬酰胺酶的反应性质[J]. 华东理工大学学报(自然科学版), 2002, 28(5): 482-486 |
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| 作者姓名： | 方波 霍红光 宋道云 江体乾 |
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| 作者单位： | 华东理工大学化学工程研究所,上海,200237 |
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| 基金项目： | 国家自然科学基金资助项目 (2 980 60 0 6) |
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| 摘 要： | 研究了乙二胺羟丙基壳聚糖固定化L-天门冬酰胺酰反应动力学性能，分别考察了固定化酶耐胃蛋白酶性能，保存条件对固定化酶活力的影响，固定化酶的米氏常数Km以及固定化酶在缓冲液中间歇催化底物和连续催化底物的反应动力学性能，结果表明，在适当的间歇和连续反应条件下，固定化酶均具有良好的水解天门冬酰胺性能和效率。
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| 关 键 词： | L－天门冬酰胺酶 固定化酶 反应动力学 壳聚糖胺基衍生物 酶活力 乙二胺羟丙基壳聚糖 |
| 文章编号： | 1006-3080(2002)05-0482-05 |
| 修稿时间： | 2001-12-11 |
Reaction Properties for the Immobilized L-asparaginase with Ethylenediamine-Hydroxypropyl Chitosan |
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| FANG Bo ,HUO Hong guang,SONG Dao yun,JIANG Ti qian. Reaction Properties for the Immobilized L-asparaginase with Ethylenediamine-Hydroxypropyl Chitosan[J]. Journal of East China University of Science and Technology, 2002, 28(5): 482-486 |
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| Authors: | FANG Bo HUO Hong guang SONG Dao yun JIANG Ti qian |
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| Affiliation: | FANG Bo *,HUO Hong guang,SONG Dao yun,JIANG Ti qian |
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| Abstract: | The reaction properties for the immobilized L aspara ginase with ethylenediamine hydroxypropyl chitosan were studied in this paper. The effects of store condition on the activity of the immobilized enzyme was inv estigated.For the immobilized enzyme,the K m value, the property of resisting protease, the bath reaction kinetics and the continuous reaction kinetics in bu ffers were studied. The results show that, under the proper react ion condition, the immobilized enzyme posseses good properties for hydrating asparagin both in batch and in continuous type. |
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| Keywords: | L asparaginase immobilized enzyme reaction kinetics aminated chitosan derivative |
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