| 紫外分光光度法测定维生素C含量 |
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| 引用本文： | 陆茜,陈凤群,万昆. 紫外分光光度法测定维生素C含量[J]. 江汉大学学报(自然科学版), 2005, 33(2): 68-69 |
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| 作者姓名： | 陆茜 陈凤群 万昆 |
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| 作者单位： | 江汉大学,化学与环境工程学院,湖北,武汉,430056;河南洛阳栾川钼业公司,河南,洛阳,471500 |
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| 摘 要： | 以甲基紫为抑制剂,加入甲基紫后维生素C的吸收峰有明显的降低。建立了一种新的光度法测定维生素C含量的方法。实验结果表明,维生素C量在1.0~50g/mL范围内服从比耳定律,回收率为98.9%,相对标准偏差3.4%,用于实际样的测定结果令人满意。
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| 关 键 词： | 维生素C测定 紫外分光光度法 甲基紫 |
| 文章编号： | 1673-0143(2005)02-0068-02 |
| 修稿时间： | 2003-10-10 |
The Determination of Ascorbate by Ultraviolet Spectrophotometry |
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| LU Qian,CHEN Feng-qun,WAN Kun. The Determination of Ascorbate by Ultraviolet Spectrophotometry[J]. Journal of Jianghan University(Natural Sciences), 2005, 33(2): 68-69 |
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| Authors: | LU Qian CHEN Feng-qun WAN Kun |
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| Affiliation: | LU Qian1,CHEN Feng-qun2,WAN Kun3 |
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| Abstract: | Using methyl violet as inhibitor,after we added methyl violet into solution theabsorbable peak of ascorbate was obviously fall. A new spectrophotometric method for determiningascorbate was established. The linear rang was 1.0 ~ 50 g mL 1. The recorery was 98.9% and RSDwas 3.4%. The method had been applied to the determination of sample with satisfactory results. |
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| Keywords: | ascorbate ultraviolet spectrophotometry methyl violet |
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