RAPD技术在罗汉松性别辨别中的应用

部分植物（包括不少濒危物种）是雌雄异株，且在非花期仅凭形态学特征难以辨别其性别；这给品种改良、植物保护工作都带来了麻烦，以罗汉松（Podocarpus macrophyllus(Thunb.)D.Don)、短叶罗汉松（Podocar、pus macrophyllus Var.maki Endl）为具体实验材料，就RAPD技术在植物性别判断方面进行了尝试，在筛选了340种随机引物后，发现其中23种引物的产物有良性的多态性；最终筛选到了可以扩增出与性别相关的特异性条带的引物P20，在这基础上对表现出多态性的RAPD产物进行了UPGMA聚类分析，观察到各样本在遗传多态性上，性别间的差异大于变种与原种间的差异，同时还对单引物、双引物的RAPD结果进行了对比。

Application of RAPD in Discrimination of Podocarpus macrophyllus''''s Sex

Podocarpus macrophyllus 's sex is hard to determine from its morphologic characteristics when they're not in blossom. This work was a trial of application of random amplified polymorphic DNA (RAPD) approach in sex discrimination of Podocarpus macrophyllus . After screening over 340 kinds of 10 bp long arbitrary primers, 23 primers were found to generate reproducible polymorphic bands, but only P20 primer could generate a common 750 bp DNA fragment with DNA samples from all the female trees. Analysis of the polymorphic bands by unweighted pair group method using arithmetic average (UPGMA) was carried out and the results showed that the disparity between male and female trees was greater than that of the same sex between different variants, Podocarpus macrophyllus (Thunb.) D.Don and Podocarpus macrophyllus Var. maki Endl . In addition, the amplification results by single primer and two primers were also compared.