大鼠肝细胞系的建立及其特性研究

从 Wistar 新生大鼠肝组织已成功获得正常肝细胞体外长期培养细胞系 NRL-84.细胞生长情况、分裂指数、染色体分析、软琼脂集落形成试验、相差显微镜及扫描电镜等多项指标观察.表明符合正常肝细胞生物学特征;用间接免疫荧光、对流免疫电泳、核酸原位杂交等检测甲胎蛋白,符合正常肝细胞的变动规律.正常新生大鼠的建立是国内首次报导,它将为进一步研究恶性肿瘤的发生发展机制提供了一个良好的实验模型和手段.

A Rat Liver Cell Line:Its Establishment and Some Characteristics Studies

A normal liver Cell line (NRL-84) derived from the new- born Wistar rat has been established in Vitro.The initial medium for cultivation was arginine-free Eagle's MEM supplemented with 10% fetal Calf serum until the 3th passages.Later it was changed to RPMI-1640 medium for continuous cultivation for more than one year (passed through 20 transfers).The cells are polygonal epithelial-like in morphology. Chromosome analysis showed a mode around 40—44 (68% frequency).AFP of this cells in the cytoplasm was assayed by immuno-fluorescent and in- situ nucleic acid hybridization methods.Counterimmunoelectrophoresis was adopted for the AFP detection in the old medium.The results showed that AFP was present both in the cytoplasm and in the medium and it gradually became weak or negative thereafter. Being the first of its kind reported in China,the establishment of normal newborn rat liver cell line will provide a satisfactory experimen- tal model and method for further studies on the mechanism of occurrence and development of malignant tumors.