毛细管胶束电动色谱-质谱联用法同时测定心脑康胶囊中3种成分含量

采用胶束电动色谱-质谱联用法同时测定心脑康胶囊中的芍药苷、葛根素、大黄素含量。该方法以未涂层石英毛细管(80cm×50μm)为分离通道,以30 mmol/L月桂酸-90 mmol/L氨水(pH值9.0)为缓冲溶液,50%异丙醇(含1 mmol/L乙酸)为鞘液,工作电压为25kV。测定结果:各组分在18 min内达到完全分离,芍药苷、葛根素、大黄素的线性范围分别为0.20～200mg/L、0.50～500mg/L、0.020～300 mg/L,检出限分别为0.020mg/L、0.050mg/L、0.0060 mg/L。样品的加标回收率在96.5%～102%之间,相对标准偏差均小于3.1%。该方法简便、快速、准确可靠,已成功应用于心脑康胶囊中芍药苷、葛根素、大黄素的含量测定。

Determination of 3 Compositions in Xinnaokang Capsules by Micellar Electrokinetic Capillary Chromatography-Electrospray Ionization Mass Spectrometry

A MEKC-MS method for the simultaneous determination of paeoniflorin,puerarin and emodin in Xinnaokang Capsules was established.The samples were separated by an uncoated capillary（80cm×50μm） on the operating voltage of 25 kV using 30 mmol/L lauric acid-90 mmol/L ammonia mixture（containing 25% acetonitrile,pH value 9.0） as the running buffer and 50% 2-Propanol 50%water mixture（containing 1 mmol/L acetic acid） as the sheath liquid.Under the optimum conditions,the baseline separation of three compounds was achieved within 18 minutes.The detection limits were 0.020 mg/L,0.050 mg/L,0.0060 mg/L for paeoniflorin,puerarin and emodin,respectively.The average recoveries of the three components were from 96.5% to 102% with all RSD less than 3.1%.The method is simple,rapid,accurate and suitable for the quality control of Xinnaokang Capsules.