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几种毛茛属植物中总黄酮的含量测定
引用本文:董南,罗兴发,陈良龙.几种毛茛属植物中总黄酮的含量测定[J].贵州大学学报(自然科学版),2007,24(2):195-197.
作者姓名:董南  罗兴发  陈良龙
作者单位:贵州大学理学院,贵州,贵阳,550025
摘    要:采用分光光度法对贵州产3种毛茛属植物石龙芮(R.sceleratus),毛茛(R.japoni-cus),禺毛茛(R.cantoniensis)的全草及不同部位中的总黄酮含量进行了测定。样品经75%乙醇提取3次,芦丁为对照品,在λ=500nm处测定,方法的平均回收率为101.4%,重现性RSD=2.26%。该法简便、准确、重现性好,适用于毛茛属植物中总黄酮的含量测定。

关 键 词:分光光度法  毛茛属  总黄酮  含量测定
文章编号:1000-5269(2007)02-0195-03
修稿时间:2006-12-22

Determination of flavonoids in several plants of ranunculus
DONG Nan,LUO Xing-fa,CHEN Liang-long.Determination of flavonoids in several plants of ranunculus[J].Journal of Guizhou University(Natural Science),2007,24(2):195-197.
Authors:DONG Nan  LUO Xing-fa  CHEN Liang-long
Institution:College of Science, Guizhou University, Guiyang 550025 ,China
Abstract:Total Flavonoids content in different parts and in the whole herb of three Ranunculus plants(R.sceleratus,R.japonicus and R.cantoniensis)was determined by Spectrophotometry.These samples were extracted 3 times with 75% ethanol,quereetin was used as standard and measured at 510nm wavelength.The average recovery was 101.4% and reproducibility was 2.26%.The methode is simple,quick,accurate and sensitive.
Keywords:Flavonoids  Ranunculus plants  Spectrophotometry
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