| 鹿茸组织和细胞原代培养和继代培养研究 |
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| 引用本文： | 徐存栓,李大卫.鹿茸组织和细胞原代培养和继代培养研究[J].河南师范大学学报(自然科学版),1990(3):102-106. |
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| 作者姓名： | 徐存栓 李大卫 |
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| 作者单位： | 河南师范大学生物系
(徐存栓，李大卫，徐军政，宛霞，高明乾，储成才，李宗义，王林嵩，尚志强)，河南师范大学生物系(高太新) |
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| 摘 要： | 本文对鹿茸皮肤及肉质部分组织块的培养及继代培养方法、从鹿茸各组织中分离细胞的方法及分离细胞的原代培养和继代培养进行了研究。由鹿茸皮肤细胞建立的细胞系已在体外培养了近四个月,传至第三十代,由鹿茸的肉质部分建立的细胞系已在体外传了二十几代。传代工作仍在继续。
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| 关 键 词： | 梅花鹿(Cervus nippon Temminck) 鹿茸 原代培养 继代培养 |
PRIMARY CULTURE AND SUBCULTURE OF PILOSE ANTLER TISSUES AND CELLS |
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| Xu Cunshuan,Li Dawei,Xu Junzheng,Wan Xia,Gao Mingqian,Chu Chengcai,Li Zongyi,Wang Lingsong,Shang Zhiqiang,Gao Taixin.PRIMARY CULTURE AND SUBCULTURE OF PILOSE ANTLER TISSUES AND CELLS[J].Journal of Henan Normal University(Natural Science),1990(3):102-106. |
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| Authors: | Xu Cunshuan Li Dawei Xu Junzheng Wan Xia Gao Mingqian Chu Chengcai Li Zongyi Wang Lingsong Shang Zhiqiang Gao Taixin |
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| Institution: | Xu Cunshuan;Li Dawei;Xu Junzheng;Wan Xia;Gao Mingqian;Chu Chengcai;Li Zongyi;Wang Lingsong;Shang Zhiqiang;Gao Taixin Department of Bioloyg |
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| Abstract: | This paper has made the preliminary study on the methods of culture and subculture of skin and flesh of piloseantler, the methods of separating cells from different parts of antler, and the primary culture and subculture of seperat-ed cells. The cells from skin of deer antler have been cultured in vitro for almost 4 months and subculture to the 30thpassage, the cells from the flesh part of antler have been subcultured 29 generations. The subculture is proceeding. |
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| Keywords: | cervus nippon Temminck pilose antler primary culture subculture |
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