Gene expression profiling related to powdery mildew resistance in wheat with the method of suppression subtractive hybridization |
| |
Authors: | Meng Luo Xiuying Kong Naxin Huo Ronghua Zhou Jizeng Jia |
| |
Institution: | (1) Institute of Crop Germplasm Resources, Chinese Academy of Agricultural Sciences, Key Laboratory of Crop Germplasm and Biotechnology, The Ministry of Agriculture, 100081 Beijing, China |
| |
Abstract: | “Bainong 3217 × Mardler” BC5F4 wheat line at the initial stage of inoculation with powdery mildew pathogen (Erysiphe graminis DC) was used to construct a suppression subtractive hybridization (SSH) cDNA library. Totally 760 ESTs were obtained through
sequencing. Similarity analysis of ESTs based on BLASTn and BLASTx with the sequences in GenBank, in combination with macroarray
differential screening, revealed that 199 ESTs of 65 kinds were known to be functionally disease resistance related. Based
on the gene expression profiling in the present study, it is postulated that salicylic acid (SA) and MAP-related signal transduction
pathways were involved in powdery mildew resistance in wheat. System acquired resistance genes were predominant in terms of
kinds and quantity. With the initiation of cell defense reaction, the genes conferring anti-oxidation substances were largely
expressed and thus cell protection mechanism was activated. Much evidence revealed that phenylpropanes metabolic pathway was
involved in phytoalexin synthesis in wheat powdery mildew resistance. Genes conferring some enzymes of structural modification
of cell walls and proteinase inhibitors inhibiting pathogen growth were also detected. The genes controlling a few proteinases
(mainly cysteine proteinase) had a considerable redundancy of expression. |
| |
Keywords: | wheat powdery mildew suppression subtractive hybridization disease resistance-related genes gene expression profiling |
本文献已被 万方数据 SpringerLink 等数据库收录! |
| 点击此处可从《中国科学通报(英文版)》浏览原始摘要信息 |
| 点击此处可从《中国科学通报(英文版)》下载免费的PDF全文 |