| 微生物酶法生产L-半胱氨酸的产酶条件优化 |
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| 引用本文： | 寇广会,怀丽华,白钢,杨文博. 微生物酶法生产L-半胱氨酸的产酶条件优化[J]. 天津科技大学学报, 2006, 21(4): 21-24 |
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| 作者姓名： | 寇广会 怀丽华 白钢 杨文博 |
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| 作者单位： | 1. 天津市工业微生物重点实验室,天津科技大学生物工程学院,天津,300457
2. 南开大学生命科学学院,天津,300071 |
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| 基金项目： | 天津市科委应用基础研究重点基金 |
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| 摘 要： | 以假单胞菌（Pseudomonas sp．）TS1138为供试菌株，对微生物酶法生产L-半胱氨酸的条件进行了初步研究。通过对产酶培养基中的碳氮源进行研究，得到了TS1138菌株产酶的最佳碳氮源分别为葡萄糖和尿素，DL—ATC的最适添加量为5g／L；通过对产酶培养基的产酶条件进行研究，得出了最适的种子接种量为10％，产酶培养基的最适初始pH为8．0，500mL三角瓶的最适装液量为40mL。
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| 关 键 词： | 假单胞菌 酶法合成 L-半胱氨酸 |
| 文章编号： | 1672-6510(2006)04-0021-04 |
| 修稿时间： | 2006-04-28 |
Optimization of Fermentation Conditions of Microbial Enzymatic Synthesis of L-cysteine |
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| KOU Guang-hui,HUAI Li-hua,BAI Gang,YANG Wen-bo. Optimization of Fermentation Conditions of Microbial Enzymatic Synthesis of L-cysteine[J]. Journal of Tianjin University of Science & Technology, 2006, 21(4): 21-24 |
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| Authors: | KOU Guang-hui HUAI Li-hua BAI Gang YANG Wen-bo |
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| Abstract: | Pseudomonas sp.TS1138 was used as the test strain to produce L-cysteine,and microbial enzymatic synthesis of L-cysteine was studied.In the medium optimization,it was found that glucose and urea were the best carbon and nitrogen source for enzyme production,respectively.Effect of DL-ATC on enzyme production was carried out,and the experimental result showed that a initial concentration of 5 g/L DL-ATC was found to be optimal for enzyme production.Effect of inoculum size,initial pH and liquid volume on enzyme production were investigated.The results indicated that the optimum inoculum size was 10%,and the optimum initial pH was 8.0,and the optimum liquid volume was 40 mL culture medium in a 500 mL shaking flask. |
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| Keywords: | Pseudomonas sp enzymatic synthesis L-cysteine |
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