Distribution of tunichrome and vanadium in sea squirt blood cells sorted by flow cytometry |
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| Authors: | E M Oltz S Pollack T Delohery M J Smith M Ojika S Lee K Kustin K Nakanishi |
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| Institution: | Department of Chemistry, Columbia University, New York, New York 10027. |
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| Abstract: | Specialized blood cells of many tunicates accumulate high concentrations of vanadium and phenolic peptide pigments called tunichromes (TC). In order to determine whether V and TC reside in the same cells, Ascidia nigra and Ascidia ceratodes blood cell subpopulations were isolated by fluorescence-activated cell sorting (flow cytometry) and chemically analyzed. V was found in the spherical, green/grey signet ring cells, and to a lesser degree in the mulberry-shaped, yellow/green morula cells (MRs), whereas free TC was detected mainly in MRs. |
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