| 逆胶束系统中脂肪酶对橄榄油的水解 |
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| 引用本文： | 茅庆成,许建和,英徐根,胡英.逆胶束系统中脂肪酶对橄榄油的水解[J].华东理工大学学报(自然科学版),1992(2). |
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| 作者姓名： | 茅庆成 许建和 英徐根 胡英 |
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| 作者单位： | 华东化工学院应用化学研究所
(茅庆成，许建和，英徐根)，华东化工学院应用化学研究所(胡英) |
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| 基金项目： | 国家自然科学基金项目(编号:2880260) |
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| 摘 要： | 在丁二酸双(2-己基已)酯磺酸钠(即AOT)/异辛烷逆胶束系统中,研究了圆柱形假丝酵母脂肪酶所催他的橄榄油水解过程。结果表明,R=H_2O]/AOT]=9.8时,水解速度最快;当R值固定时,系统的含水量越大,水解率则越高。增加橄榄油浓度,可提高水解速度,但水解率随之下降。适度搅拌能使水解速度略有提高,但会降低酶的稳定性。添加少量的甘氨酸或甘油,也会降低酶的稳定性,因而对水解不利。
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| 关 键 词： | 逆胶束 脂肪酶 橄榄油 水解 |
Hydrolysis of Olive Oil by Lipase in Reverse Micelles |
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| Mao Qingcheng,Xu Jianhe,Ying Xugen Hu Ying.Hydrolysis of Olive Oil by Lipase in Reverse Micelles[J].Journal of East China University of Science and Technology,1992(2). |
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| Authors: | Mao Qingcheng Xu Jianhe Ying Xugen Hu Ying |
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| Institution: | Research Institute of Applied Chemistry |
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| Abstract: | The hydrolysis of olive oil catalyzed by Candida cylindracea lipase in Aerosol OT (AOT)/isooctane reverse micellar solution was studied. It showed that the fastest rate of hydrosis is reached when R (H_2O]/AOT]) equals to 9.8 and once the R value is fixed, the more water this system contains, the higher degree of hydrolysis reaches. The rate of hydrolysis increases, while the degree of hydrolysis decreases, as the concentration of olive oil is raised. The rate of hydrolysis with a moderate stirring, compared to that of control, is slightly enhancod while the stability of enzyme is decreased. After small amounts of glycine or glycerol were added, the enzyme stability will also be decreased, which is disadvantageous to the hydrolysis. |
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| Keywords: | reverse micelles lipase clive oil hydrolysis |
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