| Construction of cytopathic PK-15 cell model of classical swine fever virus |
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| 作者姓名： | WUHaixiang ZHANGChuyu ZHENGCongyi WANGJiafu PANZishu LILei CAOShen YIGuanghui |
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| 作者单位： | InstituteofVirology,CollegeofLifeSciences,WuhanUniversity,Wuhart430072,China |
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| 基金项目： | 国家自然科学基金，国家重点基础研究发展计划(973计划) |
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| 摘 要： | No cytopathic effect (CPE) can be observed on classical swine fever virus (CSFV) infected cell culture in vitro. This brings an obstacle to the researches on reciprocity between CSFV and host cells. Based on the construction of full-length genomic infectious cDNA clone of Chinese CSFV standard virulent Shimen strain, partial deletion is introduced into genomic cDNA to obtain a 7.5 kb subgenomic cDNA. A new subgenomic CSFV is derived from transfection with the subgenomic cDNA on PK-15 cells pre-infected by CSFV Shimen virus. Typical CPE induced by this subgenomic virus is observed on PK-15 cells. Coexistence of wildtype and subgenomic virus in cytopathic cell culture is demonstrated by RT-PCR detection in cytopathic cells. For conclusion, the construction of cytopathic cell model exploited a new way for researches on the molecular mechanism of CSFV pathogenesis.
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| 关 键 词： | PK-15 细胞结构 病变细胞 传统猪狂热病毒 CSFV cDNA 基因克隆 |
| 收稿时间： | 2002-10-10 |
| 修稿时间： | 2003-02-26 |
Construction of cytopathic PK-15 cell model of classical swine fever virus |
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| WUHaixiang ZHANGChuyu ZHENGCongyi WANGJiafu PANZishu LILei CAOShen YIGuanghui.Construction of cytopathic PK-15 cell model of classical swine fever virus[J].Chinese Science Bulletin,2003,48(9):887-891. |
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| Authors: | Haixiang Wu Chuyu Zhang Congyi Zheng Jiafu Wang Zishu Pan Lei Li Shen Cao Guanghui Yi |
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| Institution: | e-mail: avlab@ whu.edu.cn |
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| Abstract: | No cytopathic effect (CPE) can be observed on classical swine fever virus (CSFV) infected cell culture in vitro. This brings an obstacle to the researches on reciprocity between CSFV and host cells. Based on the construction of full-length genomic infectious cDNA clone of Chinese CSFV standard virulent Shinien strain, partial deletion is introduced into genomic cDNA to obtain a 7.5 kb subgenomic cDNA. A new subgenomic CSFV is derived from transfection with the subgenomic cDNA on PK-15 cells pre-infected by CSFV Shimen virus. Typical CPE induced by this subgenomic virus is observed on PK-15 cells. Coexistence of wild-type and subgenomic virus in cytopathic cell culture is demonstrated by RT-PCR detection in cytopathic cells. For conclusion, the construction of cytopathic cell model exploited a new way for researches on the molecular mechanism of CSFV pathogenesis. |
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| Keywords: | classical swine fever virus genomic infectious clone subgenome defective interfering particle cytopathic effect |
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