褐飞虱羧酸酯酶基因cDNA片段的克隆及表达分析

利用反转录多聚酶链式反应(RT-PCR)技术克隆了褐飞虱羧酸酯酶基因编码区的cDNA片段,并进行了序列测定.结果表明,所克隆到的cDNA片段长度为396 bp,经BLAST查找比对发现,该片段所编码的氨基酸序列与来自铜绿蝇、家蝇、沟鼠、黑腹果蝇、线虫和埃及伊蚊的羧酸酯酶的片段存在高度同源性.Northern杂交分析显示,在褐飞虱取食抗性水稻后,羧酸酯酶基因表达水平明显升高.以上结果表明,羧酸酯酶基因的表达受抗性水稻的诱导,该基因在有毒化学物质解毒及增强褐飞虱对抗性水稻的耐受性方面可能起着重要作用.

Cloning and expression analyzing of carboxylesterase gene from brown planthopper Nilaparvata lugens

A RT-PCR strategy was used to clone a cDNA fragment of carboxylesterase gene from brown planthopper(Nilaparvata lugens Homoptera: Delphacidae).The cDNA fragment was sequenced and the result revealed that the length of the cDNA fragment is 396?bp in size,and the amino acid sequence deduced from the cDNA fragment has a high homology with parts of carboxylesterases from Lucilia cuprina,Musca domestica,Rattus norvegicus,Drosophila melanogaster,Caenorhabditis elegans and Aedes aegypti.Northern hybridization analysis showed that the expression of this gene rapidly increased after brown planthopper feeding on resistant rice B5.The results suggest that the expression of carboxylesterase gene in brown planthopper is induced by resistant rice,and this gene may play an important role in detoxification of rice allelochemicals and in strengthening tolerance of brown planthopper to host resistane.