哈茨木霉菌与蜡样芽孢杆菌复合使用对田间番茄根结线虫的生防效果测定

通过单一及复合使用哈茨木霉菌与蜡样芽孢杆菌，调查不同处理对大田番茄根结线虫的预防和防治效果。田间试验结果表明，连续施用两次生防菌后，两种微生物菌剂的复合使用比单一菌株对根结线虫表现出明显的预防和防治效果。45 d后两种微生物菌剂复合使用对番茄根结线虫的预防和防治效果分别达到70.2%、48.4%，根际土壤根结线虫减退率分别达到61.4%、54.9%，番茄根中根结线虫减退率分别达到75.9%、60.7%，显著高于单一菌株对根结线虫的预防和防治效果（P<0.05），研究结果为下一步研发微生物菌剂的复合使用防治番茄根结线虫提供了参考依据。

Biocontrol effect evaluation of combined Trichoderma harzianum and Bacillus cereus against root-knot nematode Meloidogyne spp. on tomatoes

Single agent Trichoderma harzianum or Bacillus cereus and combination of both were used to investigate the preventive and control effects of different treatments against root-knot nematode Meloidogyne spp. on tomatoes. The field experimental results indicated that the combination of two microbial agents showed more distinct preventive and control effects than the use of single strain after two consecutive applications of biocontrol bacteria. At 45 days after, the preventive and control effects of the combination were 70.2% and 48.4%, respectively, and the reduction rates of root-knot nematodes in rhizosphere soil were 61.4% and 54.9% and those in tomato roots were 75.9% and 60.7%. The preventive and control effects of the combination on root-knot nematodes were significantly higher compared with those observed using single strain (P<0.05). The study results provide evidence for the development of combined microbial agents to control tomato root-knot nematodes.