玉米肽螯合硒元素的制备工艺及其保肝作用研究

研究玉米肽酶解、玉米肽螯合硒元素的工艺以及玉米肽螯合硒元素的保肝作用。通过单因素、正交试验法考察优化玉米肽的酶解工艺，响应面法优化玉米肽螯合硒元素的制备工艺，并通过四氯化碳肝损伤的小鼠模型考察玉米肽螯合前后对肝脏的保护作用。当玉米蛋白质量分数为10%，在适宜的条件下加入3%菠萝蛋白酶酶解再加入2%胰蛋白酶酶解，最佳酶解条件下玉米蛋白的水解度为38.15%。在80 ℃，pH 8.05和玉米肽与硒摩尔比为2:1的条件下螯合1.5 h，玉米肽与硒元素螯合率最高（49.08%）。在保肝作用研究中，当玉米肽螯合硒元素的使用剂量为200 mg/kg时，保肝作用显著。

Preparation and hepatoprotective effect of selenium chelated by corn peptide

In this paper, the enzymatic hydrolysis of corn peptide, the chelating process of corn peptide with selenium and the protective effect of corn peptide chelate selenium on liver were studied. The enzymatic hydrolysis of corn peptide and the preparation of selenium chelate of corn peptide were investigated by single factor orthogonal test and response surface method. The liver injury model of carbon tetrachloride was established in mice to investigate the protective effect of corn peptide on liver before and after chelation. The hydrolysis degree of corn protein was 38.15% when the concentration of corn protein was 10%, 3% pineapple protease was added and 2% trypsase was added. Under the conditions of 80 ℃, at pH 8.05 and the molar ratio of corn peptide to selenium is 2:1, the chelating rate of corn peptide and selenium is the highest (49.08%). In the study of hepatoprotective effect, the liver protection effect was significant when the dosage of selenium chelated by corn peptide was 200 mg/kg.