不同产地苦杏仁药材及其饮片水提液HPLC指纹图谱研究

采用高效液相色谱法建立苦杏仁药材水提液的指纹图谱，并通过对生杏仁和燀杏仁指纹图谱的比较分析，为苦杏仁药材质量控制提供参考依据。采用Kinetex C18(2.6 μm,100 mm×4.6 mm)色谱柱，以乙腈-水为流动相进行梯度洗脱，流速0.6 mL·min-1,检测波长210 nm，柱温20 ℃，进样量10 μL。不同产地的15批苦杏仁药材共有7个共有峰，峰面积之和大于总峰面积的90%。对各产地的药材进行了相似度比较，建立了苦杏仁药材水提液的HPLC指纹图谱方法。对比生杏仁与燀杏仁的指纹图谱，发现燀杏仁中缺少1、3、7号峰，且苦杏仁苷含量明显高于生杏仁。本研究建立的指纹图谱方法可行，可以为苦杏仁药材及含苦杏仁的复方制剂质量控制提供参考。

HPLC fingerprint chromatogram of aqueous extract of Semen Armeniacae Amarae from various habitats and its decoctions

A fingerprint chromatogram of the aqueous extract of Semen Armeniacae Amarae (bitter almond) was established using high performance liquid chromatography (HPLC), and a reference was provided for the quality control of Semen Armeniacae Amarae by comparing the fingerprint chromatograms between raw and blanched almonds. A Kinetex C18(2.6 μm, 100 mm×4.6 mm) column was used, and the gradient elution was performed with acetonitrile-water as the mobile phase. The flow rate was 0.6 mL·min-1, detection wavelength 210 nm, column temperature 20 ℃, and injection volume 10 μL. There were 7 common peaks in 15 batches of Semen Armeniacae Amarae from various habitats, and the sum of the peak areas was more than 90% of the total peak area. Additionally, the medicinal materials of the examined samples were compared in terms of similarity, and the HPLC fingerprint chromatogram method of the aqueous extract of Semen Armeniacae Amarae was established. After comparing the fingerprint chromatogram between raw and blanched almonds, we found that there were no peaks 1, 3, and 7 in the latter, and the content of amygdalin in blanched almonds was significantly higher than that in raw almonds. The fingerprint chromatogram method established in this study is feasible and can provide a reference for the quality control of Semen Armeniacae Amarae medicine and preparation of compounds containing Semen Armeniacae Amarae.