| 石蒜碱对人白血病细胞K562凋亡作用的研究 |
| |
| 引用本文： | 于 淼,于 洋,刘林涛. 石蒜碱对人白血病细胞K562凋亡作用的研究[J]. 哈尔滨商业大学学报(自然科学版), 2014, 0(2): 157-160 |
| |
| 作者姓名： | 于 淼 于 洋 刘林涛 |
| |
| 作者单位： | [1] 哈尔滨商业大学生命科学与环境科学研究中心,哈尔滨150076 [2]国家教育部抗肿瘤天然药物工程研究中心,哈尔滨150076 [3]哈尔滨商业大学药物研究所博士后科研流动站,哈尔滨150076 |
| |
| 基金项目： | 黑龙江省教育厅科技项目（1211135）． |
| |
| 摘 要： | 为了研究石蒜碱对人白血病细胞K562的凋亡作用,采用MTT法检测石蒜碱对K562细胞的细胞毒作用,数据表明石蒜碱对K562细胞的IC50为16.000μmol/L.流式细胞仪检测石蒜碱对K562细胞凋亡率的影响,数据表明给药剂量越大,其凋亡率越高,凋亡率与给药剂量呈依赖关系.激光共聚焦扫描显微镜检测石蒜碱对K562细胞膜电位的影响,数据表明随着给药剂量增大,膜电位逐渐降低,且成剂量依赖关系.结果显示石蒜碱能够诱导K562细胞凋亡并使其膜电位降低.
|
| 关 键 词： | 石蒜碱 人白血病细胞K562 细胞凋亡 |
Study on apoptosis of human leukemia cell line K562 induced by lycorine |
| |
| YU Miao,YU Yang,LIU Lin-tao. Study on apoptosis of human leukemia cell line K562 induced by lycorine[J]. Journal of Harbin University of Commerce :Natural Sciences Edition, 2014, 0(2): 157-160 |
| |
| Authors: | YU Miao YU Yang LIU Lin-tao |
| |
| Affiliation: | 1. Research Center on Life Sciences and Environmental Sciences, Harbin University of Commerce, Harbin 150076, China; 2. MOE Engineering Research Center of Natural Anticancer Drugs, Harbin University of Commerce, Harbin 150076, China; 3. Postdoctoral Programme of Institute of Materia Medica, Harbin University of Commerce, Harbin 150076, China) |
| |
| Abstract: | In order to investigate the apoptosis of human leukemia cell line K 562 induced by lycorine , this paper detected the cytotoxicity of lycorine to K 562 by MTT assay , and the re-sult showed that the IC 50 was 16.000μmol/L.Flow cytometry assay showed that the apopto-sis of K562 increased in a dose-dependent manner .The confocal laser scanning microscopy observed that the membrane potential of K 562 also decreased in a dose-dependent manner . All data indicated that lycorine could induce apoptosis of K 562 and decrease its membrane potential . |
| |
| Keywords: | lycorine human leukemia cell line K562 apoptosis |
| 本文献已被 CNKI 维普 等数据库收录！ |
|
