HPLC法测定感冒退热颗粒中表告依春和连翘苷

建立HPLC法同时测定感冒退热颗粒中含表告依春和连翘苷的量,以有效控制其质量.测定条件如下:Ultimate XB-C18(5μm,4.6 mm×250 mm)为色谱柱,乙腈-0.02%磷酸水溶液(23∶77)为流动相,检测波长235 nm,流速:1.0 mL/min,柱温为35℃.结果表明,表告依春在0.010 63~0.05315μg范围内呈线性关系(R=0.999 9),加样回收率为98.50%,RSD值为0.51%(n=9);连翘苷在0.099 81~0.499 05μg范围内呈线性关系(R=0.999 9),加样回收率99.93%,RSD值为0.61%(n=9).不同生产厂家的感冒退热颗粒中含连翘苷量相差不大,但含表告依春量差异较大.本实验建立的方法简便、稳定、准确可靠,可用于感冒退热颗粒的质量控制.

Determination of epigoitrin and forsythin contents in Ganmao Tuire granules by HPLC

In order to control the quality effectively a method for determination of epigoitrin and forsythin contents simultaneously in ganmao Tuire granules was established .The samples were separated by the chromatographic column of ultimate XB -C18 （5 μm, 4.6 mm ×250 mm）.The mobile phase was acetonitrile -0.02%phosphoric acid solution （23∶77）, and the detection wavelength was at 235 nm.The flow rate was 1.0 mL/min, and the column temperature was at 35℃.Results indicated that the linear range of epigoitrin was 0.010 63-0.053 15 μg（R=0.999 9）, and the average recovery was 98.50%with RSD of 0.51%（ n=9）.The linear range of forsythin was 0.099 81~0.499 05μg （R=0.999 9）, and the average recovery was 99 .93%with RSD of 0 .61%（ n=9 ） .The forsythin content in Gan-mao Tuire granules was little different and the epigoitrin content was obviously different from different pharmaceut-ical factories .This method is simple , stable , accurate and reliable .It can be used for quality control of Ganmao Tuire granules .