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Cloning, sequencing and function ofsanB, a gene related to nikkomycin biosynthesis ofStreptomyces ansochromogenes
Authors:Wenli Li  Junyong Jia  Huarong Tan  Yongsong Hu  Zhongyan Wang
Institution:(1) Institute of Microbiology, Chinese Academy of Sciences, 100080 Beijing, China;(2) College of Life Science, Sichuan University, 610064 Chengdu, China
Abstract:A 6.0 kb DNA fragment related to nikkomycin biosynthesis was cloned from nikkomycin-producingStreptomyces ansochromogenes 7100. Sequence analysis showed that the 1.9 kbTth111 I fragment, a part of the 6.0 kb DNA fragment, contains one complete ORF designatedsanB (GenBank accession No. AF224501), which is composed of 1740 bp encoding a protein consisting of 580 amino acid residues. Its start codon is GTG at 100 bp position and stop codon is TGA at 1840-bp position. Database searching indicated that the deduced protein ofsanB is homologous to the histidinol-phosphate aminotransferase inStreptomyces coelicolor with 31% identities and 47% positives. Gene disruption was performed to study the function ofsanB. It was found that disruptants ofsanB lost the ability to synthesize nikkomycin, which reveals thatsanB is a novel gene essential for nikkomycin biosynthesis.
Keywords:Streptomyces ansochromogenes            nikkomycin biosynthesis  gene function
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