以OP-10为荧光试剂建立的高选择性Hg~(2+)检测方法

用荧光光谱法研究了烷基酚聚氧乙烯醚(OP-10)对常见金属离子的荧光识别性能,发现在适当的条件下,OP-10对Hg~(2+)呈现出高选择性荧光猝灭响应,猝灭常数为Ksv=2 112 L·mol-1.在OP-10浓度为2.0 mmol·L-1,p H值为4.1的BR缓冲溶液中,等浓度的Hg~(2+)使其在320 nm处的荧光强度降低86%.基于上述荧光猝灭作用,建立了一种新的Hg~(2+)荧光检测方法.OP-10的荧光强度与Hg~(2+)浓度在0.1×10~(-4)~4.4×10~(-4) mol·L~(-1)范围内呈良好的线性关系,线性相关系数为0.996 8.检出限为49 nmol·L-1.该荧光试剂OP-10可用于水样中Hg~(2+)的检测.

Highly selective detecting method for Hg2+based onfluorescentreagent OP-10

The recognizing property of OP-10 in the presence of common metal ions was investigated by fluorescence spectrometry.The fluorescence intensity of OP-10 was selective quenched with Hg2+ under appropriate conditions and the quenchedconstant was 2112 L·mol-1. On addition of2.0 mmol·L-1 Hg2+,the fluorescence intensity of OP-10 in BR buffered aqueous solution(pH 4.1) was quenched about 86%.Based on the above mentioned,a new method was built for fluorescence detecting Hg2+.The calibration graph is linear over the range of 0.1×10-4~4.4×10-4 mol·L-1(linearly dependent coefficient:R2= 0.9968).The detection limit was calculated to be 49 nmol·L-1.The fluorescent reagent OP-10 can detect Hg2+ in water samples.