| 大曲真核微生物群落PCR-DGGE电泳条件优化 |
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| 引用本文： | 罗惠波,侯海波,黄治国,卫春会,叶光斌.大曲真核微生物群落PCR-DGGE电泳条件优化[J].四川理工学院学报(自然科学版),2011,24(5):515-518. |
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| 作者姓名： | 罗惠波 侯海波 黄治国 卫春会 叶光斌 |
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| 作者单位： | 四川理工学院酿酒生物技术及应用四川省重点实验室,四川自贡,643000 |
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| 基金项目： | 四川省科技厅应用基础项目，四川省教育厅重大培育项目，四川理工学院引进人才项目 |
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| 摘 要： | 试验将DGGE方法应用于大曲真核微生物的研究,并对其电泳条件进行优化;对大曲真核微生物的18S rDNA优化出的DGGE电泳条件为:胶浓度8%,变性范围40%～70%,电压条件先采用200 V、4 min,再采用130 V、14 h,获得的DGGE图谱具有较好的分离效果。
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| 关 键 词： | 大曲 真核微生物 PCR-DGGE |
Optimization of PCR-DGGE Electrophoresis Conditions of Eucaryotic Microorganisms Community in Daqu |
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| LUO Hui-bo,HOU Hai-bo,HUANG Zhi-guo,WEI Chun-hui,YE Guang-bin.Optimization of PCR-DGGE Electrophoresis Conditions of Eucaryotic Microorganisms Community in Daqu[J].Journal of Sichuan University of Science & Engineering:Natural Science Editton,2011,24(5):515-518. |
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| Authors: | LUO Hui-bo HOU Hai-bo HUANG Zhi-guo WEI Chun-hui YE Guang-bin |
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| Institution: | (Liquor-making Biotechno logy & Application key Laboratory of Sichuan province,Sichuan University ofScience & Engineering,Zigong 643000,China) |
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| Abstract: | DGGE was applied to eukaryotic microbes community of Daqu in the experiment.DGGE electrophoresis conditions for 18S rDNA of eukaryotic microbes were optimized as follows： Gel concentration was 8%,denatured range was between 40% ～ 70%,electrophoresis ran 4 minutes at first as the voltage was 200V and then ran for 14 hours as the voltage was 130V.Under these conditions,satisfactory separation results could be achieved by electrophoresis. |
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| Keywords: | Daqu eukaryotic microbes PCR-DGGE |
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