| Extraction and Purification of Matrix Protein from the Nacre of Pearl Oyster Pinctada fucata |
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| 作者姓名： | 麻彩萍 张岑 聂延程 谢莉萍 张荣庆 |
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| 作者单位： | [1]Institute of Marine Biotechnology, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China [2]Protein Science Laboratory of the Ministry of Education, Tsinghua University, Beijing 100084, China |
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| 基金项目： | Supported by the National High-Tech Research and Development（863） Program of China （No. 2003AA603430） and the NationalNatural Science Foundation of China （No. 30170723） |
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| 摘 要： | A soluble matrix protein P14 with an apparent molecular mass of 14.5 kDa was isolated from fragmented nacre of pearl oysters （Pinctada fucata） treated with 10% NaOH solution to investigate the nacre matrix proteins and their effect on the CaCO3 crystal. The protein was characterized by gel exclusion chromatography and reversed-phase high performance liquid chromatography after demineralization by 10% acetic acid. The X-ray diffraction pattern of P14 crystals indicates that P14 plays an important role in nacre biomineralization. P14 can induce aragonite formation, stimulate CaCO3 crystal formation, and accelerate aragonite precipitation. Heating of the acid insoluble nacre residue, which was named conchiolin, in 10% sodium dodecyl sulfate solution supplemented with 10% β-mercaptoethanol solution for 10-20 min at about 100℃ gave two other soluble proteins having molecular masses of 19.4 kDa and 25.0 kDa. The present study suggests that these two proteins are linked to the insoluble organic matrix by disulfide bridges because the extraction yield increases when β-mercaptoethanol is added to the medium.
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| 关 键 词： | 珠母贝 牡蛎 蛋白质因子 方解石 生物特性 |
| 收稿时间： | 2004-12-09 |
| 修稿时间： | 2004-12-092005-01-14 |
Extraction and Purification of Matrix Protein from the Nacre of Pearl Oyster Pinctada fucata |
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| Caiping Ma, ົ
, Cen Zhang, , Yancheng Nie, &#x;ï«, Liping Xie, &#x;
,Rongqing Zhang, &#x;.Extraction and Purification of Matrix Protein from the Nacre of Pearl Oyster Pinctada fucata[J].Tsinghua Science and Technology,2005,10(4):499-503. |
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| Authors: | Caiping Ma ົ
Cen Zhang Yancheng Nie &#x;ï« Liping Xie &#x;
Rongqing Zhang &#x; |
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| Institution: | aInstitute of Marine Biotechnology, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China;bProtein Science Laboratory of the Ministry of Education, Tsinghua University, Beijing 100084, China |
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| Abstract: | A soluble matrix protein P14 with an apparent molecular mass of 14.5 kDa was isolated from fragmented nacre of pearl oysters (Pinctada fucata) treated with 10% NaOH solution to investigate the nacre matrix proteins and their effect on the CaCO3 crystal. The protein was characterized by gel exclusion chromatography and reversed-phase high performance liquid chromatography after demineralization by 10% acetic acid. The X-ray diffraction pattern of P14 crystals indicates that P14 plays an important role in nacre biomineralization. P14 can induce aragonite formation, stimulate CaCO3 crystal formation, and accelerate aragonite precipitation. Heating of the acid insoluble nacre residue, which was named conchiolin, in 10% sodium dodecyl sulfate solution supplemented with 10% β-mercaptoethanol solution for 10-20 min at about 100°C gave two other soluble proteins having molecular masses of 19.4 kDa and 25.0 kDa. The present study suggests that these two proteins are linked to the insoluble organic matrix by disulfide bridges because the extraction yield increases when β-mercaptoethanol is added to the medium. |
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| Keywords: | protein P14 nacre Pinctada fucata conchiolin aragonite calcite biomineralization |
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