基于电动现象的细胞光镊阱力测量

提出了一种新的细胞光阱力标定的实验方法，利用细胞电泳原理，设计并研制了一套符合用光镊测力的电动样品池系统，用它替代单光镊操作系统中的普通样品池和高精密压电位移驱动平台，可以用电压调控细胞运动速度，系统相对简单、经济．实验测量结果为：在2～18V／cm电压梯度范围内，酵母细胞的运动速度与电压梯度成正线性关系，即在皮牛量级以下，最大光阱力与光镊光源的功率成线性增加关系．测量结果表明，该方法可实现对细胞光阱力的测量，还可用来测量细胞表面电量．

Measurement for Optical Trap Force on Cells Based on Electrokinetic Phenomena

A new experimental method for calibration of optical trap force upon cells was proposed based on the theory of cells electrophoresis. An electronkinetic sample chamber system was designed instead of a common sample chamber and a costly automatism driven stage in a single beam optical tweezers system to manipulate the relative speed of a cell with respect to its liquid environment. The experimental results indicate that the yeast cell speed increases linearly with the gradient voltage ranging within 2-18 V/cm and the escape force increases linearly with the laser beam power under pN order, and the charges of the cell surface can be measured via the proposed method.