Construction and characterization of a bacterial artificial chromosome library of peach |
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| Authors: | WANG Qian ZHANG Kaichun QU Xueping JIA Jianhang LI Chuanyou JIN Demin WANG Bin |
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| Institution: | Institute of Genetics, Chinese Academy of Sciences, Beijing 100101 , China,Institute of Genetics, Chinese Academy of Sciences, Beijing 100101 , China,Institute of Genetics, Chinese Academy of Sciences, Beijing 100101 , China,Institute of Genetics, Chinese Academy of Sciences, Beijing 100101 , China,Institute of Genetics, Chinese Academy of Sciences, Beijing 100101 , China,Institute of Genetics, Chinese Academy of Sciences, Beijing 100101 , China,Institute of Genetics, Chinese Academy of Sciences, Beijing 100101 , China |
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| Abstract: | This report briefly describes the construction and characterization of a peach prunus persica (L.) Batch] Var. Jingyu bacterial artificial chromosome (BAC) library. The variety Jingyu has many important agronomic characters of stone fruits, and it is a main parent in Chinese peach breeding. After cloning of the high molecular weight peach DNA into pBeloBAC 11, we obtained over 22 000 recombinant clones. The BAC library has an average insert size of 95 kb and represents approximately 7 times peach haploid genome equivalents. After being screened with two randomly amplified polymorphic DNA markers, W4 and P20, which are linked to yellow flesh and nectarine genes of peach respectively, ten positive clones have been detected. This library is very useful for map-based cloning of peach genes and physical mapping of peach genome. |
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| Keywords: | bacterial artificial chromosome genomic library construction Prunus persica |
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