| Sensitive Determination of DNA by RLS Enhancement of Metal Ions |
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| 作者姓名： | Huang Jian-ping Chen Fang Ai Xin-ping He Zhi-ke . College of Chemistry and Molecular Sciences Wuhan University Wuhan Hubei China . |
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| 作者单位： | Huang Jian-ping 1，2，Chen Fang 1，Ai Xin-ping 1，He Zhi-ke 1 1. College of Chemistry and Molecular Sciences，Wuhan University，Wuhan 430072，Hubei，China; 2. Department of Environmental Engineering，Hubei Agricultural College，Jingzhou 434025，Hubei，China |
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| 基金项目： | SupportedbyNationalNaturalScienceFoundationofChina(2 0 2 750 2 8)andtheNationalKeyBasicResearchandDevelopmentProgram (973Program 2 0 0 2CB2 1 1 80 0 ) |
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| 摘 要： | 0 IntroductionThequantitativeanalysisofnucleicacids,especiallythemi cro determinationofnucleicacids,isbecomingmoreandmoreimportantinmanybiologicalstudies.Recently ,apromisingspectraltechnique ,whichwasbasedonthemeasurementofen hancedresonancelightscattering (RLS) 1 ,2 ] ,hasgivenrisetostronginterestbyanalystsandbiochemistsfornucleicacidsandproteinassay3 9] .Uptonow ,manykindscompoundshavebeenfoundRLSenhancementwhilebindingtoDNA ,andallthesecompoundsarecharacterizedofpositivechargewhicha…
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| 关 键 词： | 金属离子 共振光散射 RLS 脱氧核糖核酸 DNA |
| 收稿时间： | 29 May 2003 |
Sensitive determination of DNA by RLS enhancement of metal ions |
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| Huang?Jian-ping,Chen?Fang,Ai?Xin-ping,He?Zhi-ke.Sensitive Determination of DNA by RLS Enhancement of Metal Ions[J].Wuhan University Journal of Natural Sciences,2004,9(2):234-238. |
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| Authors: | Huang Jian-ping Chen Fang Ai Xin-ping He Zhi-ke |
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| Institution: | (1) College of Chemistry and Molecular Sciences, Wuhan University, 430072 Wuhan, Hubei, China;(2) Department of Environmental Engineering, Hubei Agricultural College, 434025 Jingzhou, Hubei, China |
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| Abstract: | The interactions between metal ions and DNA have been studied by the resonance light scattering (RLS) spectra. In the acidic
condition, the RLS signals of metal ions, especially the transition metal ions in group IB and IIB, were increased by DNA.
And it is found that the enhancement of RLS signals is linear to the concentration of DNA, so the RLS method for DNA determination
was proposed in the presence of Cu2+. On the optimum conditions, the linear range and the detect limit of ctDNA is 4×10−8–4×10−6 g· mL−1 and 1. 13×10−8 g·mL−1, respectively. The proposed method is successfully applied to determine the extracted plasmid DNA ofBacillus subtilis DB104.
Foundation item: Supported by National Natural Science Foundation of China (20275028) and the National Key Basic Research and Development
Program (973 Program 2002CB211800).
Biography: Huang Jian-ping(1964-), male, Associate professor, research direction: bioanalysis. |
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| Keywords: | resonance light scattering DNA metal ions |
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