微流控毛细管电泳-流动注射联用技术在分离和测定中药制剂中麻黄碱与伪麻黄碱的应用

采用较短的毛细管作为分离通道,建立了一种微流控毛细管电泳和流动注射联用的分离测定麻黄碱和伪麻黄碱的新体系.该体系由一个H-型微芯片接口、一个水平放置的毛细管(作为分离通道)、两个竖直放置的Tygon管(作为阳极和阴极的电解质流通储液槽)组成.在最佳实验条件下,麻黄碱和伪麻黄碱标准品的进样频率可达到60 h-1,且完全基线分离,重现性良好,检测限(S/N=3)分别为:麻黄1.77 μg/mL,伪麻黄2.03 μg/mL.该微流控体系已用于3种含有麻黄碱和伪麻黄碱的市售药品的测定,结果令人满意.

Micro-fluidic capillary electrophoresis system with flow injection sample introduction applied to separation and determination of ephedrine and pseudoephedrine

In this work, a microfluidic capillary electrophoresis system coupled to a flow injection sample introduction system using short capillary column as separation channel is described. This device contains an H-shaped microchip fixed on a planar plastic base, which utilizes a horizontal separation capillary with tubular side arms on each end that serve as inlet and outlet flow-through electrode reservoirs. Continuous FI introduction of a series of samples containing a standard mixture of ephedrine and pseudoephedrine allows a throughput rate up to 60 h-1 with complete baseline separation and high precision. The limits of detection (S/N=3) are 1.77 μg/mL for ephedrine and 2.03 μg/mL for pseudoephedrine, respectively. The microfluidic system has been applied to analyzing, for the first time, three commercial pharmaceutical preparations containing ephedrine or pseudoephedrine, and the results are satisfactory.