Sequencing of p35 gene fromHeliothis armigera polyhedrosis virus and its homologous comparison |
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Authors: | Wang Ye-fu Qi Yi-peng Li Zhi-da Zhu Ying Su fei |
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Institution: | (1) College of Life Sciences, Wuhan University, 430072 Wuhan, China |
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Abstract: | Using p35 gene primers of AcNPV, about 1 kb fragment was obtained by PCR from HaNPV DNA and was sequenced thereafter. It has
a full reading frame encode 299 amino acids. Sharing identity of 94% in nucleotides and 84% in predict amino-acids with AcNPV,
a apoptosis inhibiting gene was found. According to comparison of p35 genes in five kinds of baculovirus, it is found that
AcNPV, TnNPV and BmNPV share the most intimate blood relation. HaNPV is near the above three species. LsNPV is little remote
from them. This result reconfirmed those we have done with gp37 genes and vp39 genes. It is more accurate to use conserved
gene for species division than that of the serological identification.
Biography: WANG Ye-fu(1962-), male, Ph D. |
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Keywords: | molecular evolution homologous comparison PCR amplification |
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