外源DNA导入麦类作物结实率的研究

以普通小麦和硬粒小麦为受体、以亲缘关系不同的7个材料作供体，研究了花粉管通道法转化植株的当代结实率。结果表明：（1）受体为普通小麦时，在授粉后4-8h转化植株的结实率较高；受体为硬粒小麦时，时间上较为分散。（2）不同供体的转化植株的结实率差异较大；不同浓度的外源DNA导入后的当代结实率也存在较大的差异。（3）在不同的缓冲系统中，PBS系统的转化植株的结实率高于SSC和TE系统。由此可知，在不同条件下，利用花粉管通道法转化植株，其结实率受到一定的影响。

Study on Seed-bearing Ratio of Wheat & Durum Introduced by Exogenous DNA via Pollen Tube Pathway

Wheat and durum were taken as acceptors and the DNAs of 7 different plant materials were taken as donors, authors had studied seed-bearing ratio of modern generation plants transformed by pollen tube pathway. The results were shown as follow. (1) The seed-bearing ratio of wheat as an acceptor were highest on plant transformed from 4 to 8 hour after given pollen, but the seed-bearing ratio of durum became dispersing at different time. (2)There were important differences of those seed-bearing ratio among different donor and among exogenous DNA at different consistency. (3) The seed-bearing ratio of plant introduced by PBS DNA buffer system were higher than those of SSC and TE buffers. There were differences of seed-bearing ratio on plant introduced at different condition.