hShroom1 links a membrane bound protein to the actin cytoskeleton |
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| Authors: | D. E. Dye S. Karlen B. Rohrbach O. Staub L. R. Braathen K. A. Eidne D. R. Coombe |
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| Affiliation: | (1) School of Biomedical Sciences, Curtin University of Technology, Molecular Immunology Group, Level 5 MRF Building, Rear 50 Murray Street, Perth, 6000, Australia;(2) Dermatological Clinic, Inselspital, Berne, Switzerland;(3) Institute of Pharmacology and Toxicology, University of Lausanne, Lausanne, Switzerland;(4) Western Australian Institute of Medical Research, Centre for Medical Research, The University of Western Australia, Perth, Australia |
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| Abstract: | hShroom1 (hShrm1) is a member of the Apx/Shroom (Shrm) protein family and was identified from a yeast two-hybrid screen as a protein that interacts with the cytoplasmic domain of melanoma cell adhesion molecule (MCAM). The characteristic signature of the Shrm family is the presence of a unique domain, ASD2 (Apx/Shroom domain 2). mRNA analysis suggests that hShrm1 is expressed in brain, heart, skeletal muscle, colon, small intestine, kidney, placenta and lung tissue, as well a variety of melanoma and other cell lines. Co-immunoprecipitation and bioluminescence resonance energy transfer (BRET) experiments indicate that hShrm1 and MCAM interact in vivo and by immunofluorescence microscopy some co-localization of these proteins is observed. hShrm1 partly co-localises with β-actin and is found in the Triton X-100 insoluble fraction of melanoma cell extracts. We propose that hShrm1 is involved in linking MCAM to the cytoskeleton. D. E. Dye, S. Karlen: These authors contributed equally to this work. Received 09 October 2008; received after revision 23 November 2008; accepted 09 December 2008 |
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| Keywords: | KeywordHeading" >. Shroom Apx MCAM CD146 melanoma |
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